Source:http://linkedlifedata.com/resource/pubmed/id/14975926
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
2004-5-18
|
| pubmed:abstractText |
Hypertrophic growth of cardiac muscle is dependent on activation of the PKC-epsilon isoform. To define the effectors of PKC-epsilon involved in growth regulation, recombinant adenoviruses were used to overexpress either wild-type PKC-epsilon (PKC-epsilon/WT) or dominant negative PKC-epsilon (PKC-epsilon/DN) in neonatal rat cardiocytes. PKC-epsilon/DN inhibited acute activation of PKC-epsilon produced in response to phorbol ester and reduced ERK1/2 activity as measured by the phosphorylation of p42 and p44 isoforms. The inhibitory effects were specific to PKC-epsilon because PKC-epsilon/DN did not prevent translocation of either PKC-alpha or PKC-delta. Overexpression of PKC-epsilon/DN blunted the acute increase in ERK1/2 phorphorylation induced by the alpha(1)-adrenergic agonist phenylephrine (PE ). Inhibition of PKC-delta with rottlerin potentiated the effects of PE on ERK1/2 phosphorylation. PKC-epsilon/DN adenovirus also blocked cardiocyte growth as measured after 48 h of PE treatment, although the multiplicity of infection was lower than that required to block acute ERK1/2 activation. PE activated p38 mitogen-activated protein kinase as measured by its phosphorylation, but the response was not blocked by PKC inhibitors or by overexpression of PKC-epsilon/DN. Taken together, these studies show that the hypertrophic agonist PE regulates ERK1/2 activity in cardiocytes by a pathway dependent on PKC-epsilon and that PE-induced growth is mediated by PKC-epsilon.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cardiotonic Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/PRKCD protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/PRKCE protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Phenylephrine,
http://linkedlifedata.com/resource/pubmed/chemical/Prkcd protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Prkce protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C-delta,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C-epsilon
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jun
|
| pubmed:issn |
0363-6135
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
286
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
H2195-203
|
| pubmed:dateRevised |
2009-11-19
|
| pubmed:meshHeading |
pubmed-meshheading:14975926-Adenoviridae,
pubmed-meshheading:14975926-Animals,
pubmed-meshheading:14975926-Cardiotonic Agents,
pubmed-meshheading:14975926-Cell Division,
pubmed-meshheading:14975926-Cells, Cultured,
pubmed-meshheading:14975926-Gene Transfer Techniques,
pubmed-meshheading:14975926-Humans,
pubmed-meshheading:14975926-MAP Kinase Signaling System,
pubmed-meshheading:14975926-Mitogen-Activated Protein Kinase 1,
pubmed-meshheading:14975926-Mitogen-Activated Protein Kinase 3,
pubmed-meshheading:14975926-Mitogen-Activated Protein Kinases,
pubmed-meshheading:14975926-Myocytes, Cardiac,
pubmed-meshheading:14975926-Phenylephrine,
pubmed-meshheading:14975926-Protein Kinase C,
pubmed-meshheading:14975926-Protein Kinase C-delta,
pubmed-meshheading:14975926-Protein Kinase C-epsilon,
pubmed-meshheading:14975926-Rats
|
| pubmed:year |
2004
|
| pubmed:articleTitle |
PKC-epsilon regulation of extracellular signal-regulated kinase: a potential role in phenylephrine-induced cardiocyte growth.
|
| pubmed:affiliation |
Cardiology Division, Department of Medicine, Gazes Cardiac Research Institute, Medical University of South Carolina, 303 Thurmond Bldg., 114 Doughty Street, Charleston, SC 29403, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|