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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2004-2-19
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pubmed:abstractText |
A methanol extract of chaste-tree berry (Vitex agnus-castus L.) was tested for its ability to displace radiolabeled estradiol from the binding site of estrogen receptors alpha (ERalpha) and beta (ERbeta). The extract at 46 +/- 3 microg/ml displaced 50% of estradiol from ERalpha and 64 +/- 4 microg/ml from ERbeta. Treatment of the ER+ hormone-dependent T47D:A18 breast cancer cell line with the extract induced up-regulation of ERbeta mRNA. Progesterone receptor (PR) mRNA was upregulated in the Ishikawa endometrial cancer cell line. However, chaste-tree berry extract did not induce estrogen-dependent alkaline phosphatase (AP) activity in Ishikawa cells. Bioassay-guided isolation, utilizing ER binding as a monitor, resulted in the isolation of linoleic acid as one possible estrogenic component of the extract. The use of pulsed ultrafiltration liquid chromatography-mass spectrometry, which is an affinity-based screening technique, also identified linoleic acid as an ER ligand based on its selective affinity, molecular weight, and retention time. Linoleic acid also stimulated mRNA ERbeta expression in T47D:A18 cells, PR expression in Ishikawa cells, but not AP activity in Ishikawa cells. These data suggest that linoleic acid from the fruits of Vitex agnus-castus can bind to estrogen receptors and induce certain estrogen inducible genes.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Estrogen Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Estrogen Receptor alpha,
http://linkedlifedata.com/resource/pubmed/chemical/Estrogen Receptor beta,
http://linkedlifedata.com/resource/pubmed/chemical/Linoleic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Extracts,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Estrogen,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Progesterone
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0944-7113
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pubmed:author |
pubmed-author:BoltonJ LJL,
pubmed-author:BurdetteJ EJE,
pubmed-author:DenkWW,
pubmed-author:FarnsworthN RNR,
pubmed-author:FongH H SHH,
pubmed-author:LiuJJ,
pubmed-author:MahadyGG,
pubmed-author:NikolicDD,
pubmed-author:PezzutoJ MJM,
pubmed-author:SchlechtS MSM,
pubmed-author:SunYY,
pubmed-author:ZhengWW,
pubmed-author:van BreemenR BRB
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pubmed:issnType |
Print
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pubmed:volume |
11
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
18-23
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pubmed:dateRevised |
2009-6-9
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pubmed:meshHeading |
pubmed-meshheading:14974442-Breast Neoplasms,
pubmed-meshheading:14974442-Cell Line, Tumor,
pubmed-meshheading:14974442-DNA Primers,
pubmed-meshheading:14974442-Endometrial Neoplasms,
pubmed-meshheading:14974442-Estrogen Antagonists,
pubmed-meshheading:14974442-Estrogen Receptor alpha,
pubmed-meshheading:14974442-Estrogen Receptor beta,
pubmed-meshheading:14974442-Female,
pubmed-meshheading:14974442-Fruit,
pubmed-meshheading:14974442-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:14974442-Humans,
pubmed-meshheading:14974442-Linoleic Acid,
pubmed-meshheading:14974442-Neoplasms, Hormone-Dependent,
pubmed-meshheading:14974442-Phytotherapy,
pubmed-meshheading:14974442-Plant Extracts,
pubmed-meshheading:14974442-RNA, Messenger,
pubmed-meshheading:14974442-Receptors, Estrogen,
pubmed-meshheading:14974442-Receptors, Progesterone,
pubmed-meshheading:14974442-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:14974442-Vitex
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pubmed:year |
2004
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pubmed:articleTitle |
Isolation of linoleic acid as an estrogenic compound from the fruits of Vitex agnus-castus L. (chaste-berry).
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pubmed:affiliation |
Department of Medicinal Chemistry and Pharmacognosy, UIC/NIH Center for Botanical Dietary Supplements Research, College of Pharmacy, University of Illinois at Chicago, Chicago, Illinois 60612, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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