Source:http://linkedlifedata.com/resource/pubmed/id/14973539
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
2004-2-19
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| pubmed:abstractText |
In this study, we investigated to what extent the stability and transduction capacity of polyplexed DNA can be improved by optimizing the condensing peptide sequence. We have synthesized a small library of cationic peptides, at which the lysine/arginine ratio and the cation charge were varied. All peptides were able to compact DNA, at which polyplexes of short lysine-rich sequences were considerably larger than those of elongated or arginine-rich peptides (GM102 and GM202). In addition, the arginine-rich peptides GM102 and GM202 rendered the polyplexes resistant to plasma incubation or DNase I-mediated digestion. While all peptides were found to improve the transfection efficiency in HepG2 cells, only the GM102- and GM202-derived polyplexes could be specifically targeted to HepG2 cells by incorporation of a ligand-derivatized YKAK(8)WK peptide. We propose that GM102 and GM202 combine the advantage of small condensing peptides to give small-sized polyplexes with the superior stability of condensing polymers, which makes GM102 and GM202 excellent candidates for future in vivo gene therapy studies.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Mar
|
| pubmed:issn |
0969-7128
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
11
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
457-64
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:14973539-Amino Acid Sequence,
pubmed-meshheading:14973539-Animals,
pubmed-meshheading:14973539-Arginine,
pubmed-meshheading:14973539-Chemistry, Physical,
pubmed-meshheading:14973539-Deoxyribonuclease I,
pubmed-meshheading:14973539-Drug Stability,
pubmed-meshheading:14973539-Gene Targeting,
pubmed-meshheading:14973539-Gene Transfer Techniques,
pubmed-meshheading:14973539-Genetic Vectors,
pubmed-meshheading:14973539-Humans,
pubmed-meshheading:14973539-Male,
pubmed-meshheading:14973539-Mice,
pubmed-meshheading:14973539-Mice, Inbred C57BL,
pubmed-meshheading:14973539-Molecular Sequence Data,
pubmed-meshheading:14973539-Oligopeptides,
pubmed-meshheading:14973539-Peptide Fragments,
pubmed-meshheading:14973539-Physicochemical Phenomena,
pubmed-meshheading:14973539-Tissue Distribution,
pubmed-meshheading:14973539-Transduction, Genetic,
pubmed-meshheading:14973539-Transfection
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| pubmed:year |
2004
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| pubmed:articleTitle |
Stable polyplexes based on arginine-containing oligopeptides for in vivo gene delivery.
|
| pubmed:affiliation |
Division of Biopharmaceutics, Gorlaeus Laboratories, Leiden/Amsterdam Center for Drug Research, Leiden University, Leiden, The Netherlands.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|