Linked Life Data

14973283

Source:http://linkedlifedata.com/resource/pubmed/id/14973283

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2004-2-19
pubmed:abstractText
Transcription factors of the auxin response factor (ARF) family have been implicated in auxin-dependent gene regulation, but little is known about the functions of individual ARFs in plants. Here, interaction assays, expression studies and combinations of multiple loss- and gain-of-function mutants were used to assess the roles of two ARFs, NONPHOTOTROPIC HYPOCOTYL 4 (NPH4/ARF7) and MONOPTEROS (MP/ARF5), in Arabidopsis development. Both MP and NPH4 interact strongly and selectively with themselves and with each other, and are expressed in vastly overlapping domains. We show that the regulatory properties of both genes are far more related than suggested by their single mutant phenotypes. NPH4 and MP are capable of controlling both axis formation in the embryo and auxin-dependent cell expansion. Interaction of MP and NPH4 in Arabidopsis plants is indicated by their joint requirement in a number of auxin responses and by synergistic effects associated with the co-overexpression of both genes. Finally, we demonstrate antagonistic interaction between ARF and Aux/IAA gene functions in Arabidopsis development. Overexpression of MP suppresses numerous defects associated with a gain-of-function mutation in BODENLOS (BDL)/IAA12. Together these results provide evidence for the biological relevance of ARF-ARF and ARF-Aux/IAA interaction in Arabidopsis plants and demonstrate that an individual ARF can act in both invariantly programmed pattern formation as well as in conditional responses to external signals.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0950-1991
pubmed:author
pubmed:issnType
Print
pubmed:volume
131
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1089-100
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:14973283-Arabidopsis, pubmed-meshheading:14973283-Arabidopsis Proteins, pubmed-meshheading:14973283-Base Sequence, pubmed-meshheading:14973283-Body Patterning, pubmed-meshheading:14973283-DNA, Plant, pubmed-meshheading:14973283-DNA-Binding Proteins, pubmed-meshheading:14973283-Gene Expression Regulation, Developmental, pubmed-meshheading:14973283-Gene Expression Regulation, Plant, pubmed-meshheading:14973283-Genes, Plant, pubmed-meshheading:14973283-In Situ Hybridization, pubmed-meshheading:14973283-Indoleacetic Acids, pubmed-meshheading:14973283-Mutation, pubmed-meshheading:14973283-Phenotype, pubmed-meshheading:14973283-Plants, Genetically Modified, pubmed-meshheading:14973283-RNA, Messenger, pubmed-meshheading:14973283-RNA, Plant, pubmed-meshheading:14973283-Transcription Factors, pubmed-meshheading:14973283-Two-Hybrid System Techniques
pubmed:year
2004
pubmed:articleTitle
Overlapping and non-redundant functions of the Arabidopsis auxin response factors MONOPTEROS and NONPHOTOTROPIC HYPOCOTYL 4.
pubmed:affiliation
McGill University, Biology Department, 1205 Docteur Penfield Avenue, Montréal, Québec H3A 1B1, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't