Linked Life Data

14971693

Source:http://linkedlifedata.com/resource/pubmed/id/14971693

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2004-2-19
pubmed:abstractText
Parasite genotyping by a polymerase chain reaction was used to distinguish recrudescent from newly acquired Plasmodium falciparum infections in 50 of 160 Nigerian children taking part in a chloroquine efficacy study in Ibadan, Nigeria. A finger prick blood sample was taken from each child before and after treatment to identify recrudescent parasites. By investigating allelic variation in three polymorphic antigen loci, merozoite surface protein-1 (MSP-1), MSP-2, and glutamate-rich protein (GLURP), we determined parasite diversity in the population and in the infected host. DNA from pretreatment and post-treatment samples from 47 of the 50 patients who failed therapy was successfully amplified by the PCR. The MSP-1, MSP-2, and GLURP genotypes in all samples showed extensive diversity, indicating polyclonal infections. The average number of clones per infection in pre-treatment sample was 2.5 with MSP-1, 4.9 with MSP-2, and 2 with GLURP. The extent of multiplicity decreased significantly (P = 0.016) in posttreatment samples. Multiplicity of infection and initial parasite density were not age dependent. Comparison of the variant alleles in pretreatment and post-treatment samples of each patient indicates that 26 of the 47 children had genuinely recrudescent disease. Conversely, post-treatment samples from five children showed completely new genotypes, indicating either a previously sequestered population of parasites or a newly acquired infection. Overall, this study has shown the diversity and complexity of P. falciparum population in Ibadan, Nigeria. The study has also shown the dynamics of P. falciparum infections in this population before and after chloroquine treatment in an area of high malaria transmission.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0002-9637
pubmed:author
pubmed:issnType
Print
pubmed:volume
70
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
20-6
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:14971693-Animals, pubmed-meshheading:14971693-Antigens, Protozoan, pubmed-meshheading:14971693-Antimalarials, pubmed-meshheading:14971693-Child, pubmed-meshheading:14971693-Child, Preschool, pubmed-meshheading:14971693-Chloroquine, pubmed-meshheading:14971693-DNA, Protozoan, pubmed-meshheading:14971693-Drug Resistance, pubmed-meshheading:14971693-Genetic Variation, pubmed-meshheading:14971693-Humans, pubmed-meshheading:14971693-Infant, pubmed-meshheading:14971693-Malaria, Falciparum, pubmed-meshheading:14971693-Merozoite Surface Protein 1, pubmed-meshheading:14971693-Nigeria, pubmed-meshheading:14971693-Plasmodium falciparum, pubmed-meshheading:14971693-Polymerase Chain Reaction, pubmed-meshheading:14971693-Protozoan Proteins, pubmed-meshheading:14971693-Recurrence, pubmed-meshheading:14971693-Treatment Failure
pubmed:year
2004
pubmed:articleTitle
Molecular analysis of Plasmodium falciparum recrudescent malaria infections in children treated with chloroquine in Nigeria.
pubmed:affiliation
Malaria Research Laboratories, Postgraduate Institute of Medical Research and Training, College of Medicine, University of Ibadan, Ibadan, Nigeria.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't