Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2004-2-18
pubmed:abstractText
Benign as well as malignant tumour tissues of the breast demonstrate higher fluorescence intensity (FI) than normal breast tissue after application of a photosensitiser. As a follow-up study, we evaluated the FI of metastatic sentinel lymph nodes and metastatic axillary lymph nodes compared to nonmetastatic sentinel and axillary lymph nodes in patients with breast cancer. In all, 11 patients received 30 mg 5-aminolevulinic acid (ALA) kg(-1) bodyweight orally 3 h prior to surgery. The sentinel lymph node was marked with Nanocoll preoperatively and with a blue dye intraoperatively. Tumour excision, excision of the sentinel lymph node and an axillary lymph node dissection were performed during the same surgical session. The operation site was illuminated with blue light (400 nm) to obtain macroscopic tissue characterisation of fluorescence. Tissue samples were stored protected from light, and analysed using a fluorescence microscope. Results were correlated with histopathology. In all, 14 sentinel lymph nodes, seven axillary lymph nodes and seven primary tumours were analysed. Metastatic sentinel lymph nodes demonstrated a statistically significant higher FI than nonmetastatic sentinel lymph nodes (2630 vs 526, P<0.0001). The FI of metastatic sentinel lymph nodes, of metastatic axillary lymph nodes and of the primary tumour were comparably high, and were statistically significantly higher compared to the normal mammary tissue. Intraoperatively, only in a few cases, it was possible to recognise the metastatic sentinel lymph node macroscopically with blue light. Our study indicates that photodynamic diagnosis with ALA has a potential in the diagnosis and detection of the sentinel lymph node in patients with breast cancer, and is worth to be further investigated and developed for intraoperative photodynamic diagnosis and possibly therapy.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/14970857-10938787, http://linkedlifedata.com/resource/pubmed/commentcorrection/14970857-11136571, http://linkedlifedata.com/resource/pubmed/commentcorrection/14970857-11139309, http://linkedlifedata.com/resource/pubmed/commentcorrection/14970857-11255332, http://linkedlifedata.com/resource/pubmed/commentcorrection/14970857-11298571, http://linkedlifedata.com/resource/pubmed/commentcorrection/14970857-11477585, http://linkedlifedata.com/resource/pubmed/commentcorrection/14970857-11573228, http://linkedlifedata.com/resource/pubmed/commentcorrection/14970857-12604732, http://linkedlifedata.com/resource/pubmed/commentcorrection/14970857-1327020, http://linkedlifedata.com/resource/pubmed/commentcorrection/14970857-7745060, http://linkedlifedata.com/resource/pubmed/commentcorrection/14970857-8778526, http://linkedlifedata.com/resource/pubmed/commentcorrection/14970857-8942497, http://linkedlifedata.com/resource/pubmed/commentcorrection/14970857-9126192, http://linkedlifedata.com/resource/pubmed/commentcorrection/14970857-9612195, http://linkedlifedata.com/resource/pubmed/commentcorrection/14970857-9825692
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0007-0920
pubmed:author
pubmed:issnType
Print
pubmed:day
23
pubmed:volume
90
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
805-9
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Photodynamic detection of diseased axillary sentinel lymph node after oral application of aminolevulinic acid in patients with breast cancer.
pubmed:affiliation
Department of Obstetrics and Gynaecology, University Hospital Bern, Inselspital, Effingerstrasse 102, Bern CH-3010, Switzerland. ka.frei@gmx.ch
pubmed:publicationType
Journal Article, Clinical Trial