Source:http://linkedlifedata.com/resource/pubmed/id/14966463
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2004-2-17
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| pubmed:abstractText |
The multifunctional cytokine interleukin-1beta (IL-1beta) plays a central role in the body's immune and inflammatory responses. The mechanism of IL-1beta on thrombocytosis and megakaryocytopoiesis has remained controversial. In previous reports, we have demonstrated the expression of IL-1 receptors (IL-1RI and IL-1RII) and enhancing effects of IL-1beta on primary human megakaryocytic (MK) cells. In this study, we investigated the possible direct effects of IL-1beta on the expression of thrombopoietin (TPO) and transcription factors c-Jun, c-Fos, GATA-1, and p45 nuclear factor-E2 (NF-E2) in MK cell lines CHRF and Meg-01. Our results demonstrated that IL-1beta up-regulated messenger RNA (mRNA) and protein expressions of these transcription factors in a dose- and time-dependent manner. In CHRF cells, mRNA: c-Jun [3.4-fold, peaked at 15 minutes], c-Fos [4.2-fold, 15 minutes], GATA-1 [4.0-fold, 60 minutes], NF-E2 [3.2-fold, 120 minutes] and protein expression: c-Jun [3.0-fold, 30 minutes], c-Fos [1.7-fold, 30 minutes], GATA-1 [11.5-fold, 60 minutes], NF-E2 [12.5-fold, 120 minutes] were evidently enhanced after treatment with IL-1beta. The response to IL-1beta was consistent in the total cell and nuclear extracts and was significantly reduced by pretreatment with actinomycin D or cycloheximide. An IL-1-receptor antagonist (IL-1RA) inhibited the stimulatory effects of IL-1beta on these transcription factors by as much as 78%. TPO expression was increased by more than 9.9-fold on stimulation with IL-1beta. A TPO-neutralizing antibody did not significantly reduce the effects of IL-1beta. We conclude that IL-1beta up-regulates the expression of TPO, c-Jun, c-Fos, GATA-1, and NF-E2 in MK cells. The mechanism might be mediated by IL-1beta receptors and require transcription or protein synthesis. The direct involvement of IL-1beta in the MK lineage may provide an explanation for the phenomenon of thrombocytosis during inflammatory responses.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cycloheximide,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Dactinomycin,
http://linkedlifedata.com/resource/pubmed/chemical/Erythroid-Specific DNA-Binding...,
http://linkedlifedata.com/resource/pubmed/chemical/GATA1 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/GATA1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/NF-E2 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/NF-E2 Transcription Factor, p45...,
http://linkedlifedata.com/resource/pubmed/chemical/NFE2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-fos,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-jun,
http://linkedlifedata.com/resource/pubmed/chemical/Thrombopoietin,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
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| pubmed:issn |
0022-2143
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
143
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
75-88
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:14966463-Base Sequence,
pubmed-meshheading:14966463-Blotting, Southern,
pubmed-meshheading:14966463-Cell Line,
pubmed-meshheading:14966463-Cycloheximide,
pubmed-meshheading:14966463-DNA Primers,
pubmed-meshheading:14966463-DNA-Binding Proteins,
pubmed-meshheading:14966463-Dactinomycin,
pubmed-meshheading:14966463-Erythroid-Specific DNA-Binding Factors,
pubmed-meshheading:14966463-GATA1 Transcription Factor,
pubmed-meshheading:14966463-Gene Expression Regulation,
pubmed-meshheading:14966463-Humans,
pubmed-meshheading:14966463-Interleukin-1,
pubmed-meshheading:14966463-Kinetics,
pubmed-meshheading:14966463-Megakaryocytes,
pubmed-meshheading:14966463-NF-E2 Transcription Factor,
pubmed-meshheading:14966463-NF-E2 Transcription Factor, p45 Subunit,
pubmed-meshheading:14966463-Proto-Oncogene Proteins c-fos,
pubmed-meshheading:14966463-Proto-Oncogene Proteins c-jun,
pubmed-meshheading:14966463-Thrombopoietin,
pubmed-meshheading:14966463-Transcription Factors
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| pubmed:year |
2004
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| pubmed:articleTitle |
Interleukin-1beta up-regulates the expression of thrombopoietin and transcription factors c-Jun, c-Fos, GATA-1, and NF-E2 in megakaryocytic cells.
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| pubmed:affiliation |
Department of Pediatrics, Prince of Wales Hospital, Chinese University of Hong Kong, People's Republic of China.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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