Linked Life Data

14966141

Source:http://linkedlifedata.com/resource/pubmed/id/14966141

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
16
pubmed:dateCreated
2004-4-12
pubmed:databankReference
pubmed:abstractText
Little is known about the specific signaling roles of Rap2, a Ras family small GTP-binding protein. In a search for novel Rap2-interacting proteins by the yeast two-hybrid system, we isolated isoform 3 of the human mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), a previously described but uncharacterized isoform. Other isoforms of MAP4K4 in humans and mice are known as hematopoietic progenitor kinase (HPK)/germinal center kinase (GCK)-like kinase and Nck-interacting kinase, respectively. MAP4K4 belongs to the STE20 group of protein kinases and regulates c-Jun N-terminal kinase (JNK). MAP4K4 interacted with Rap2 through its C-terminal citron homology domain but did not interact with Rap1 or Ras. Interaction with Rap2 required the intact effector region of Rap2. MAP4K4 interacted preferentially with GTP-bound Rap2 over GDP-bound Rap2 in vitro. In cultured cells, MAP4K4 colocalized with Rap2, while a mutant MAP4K4 lacking the citron homology domain failed to do so. Furthermore, Rap2 enhanced MAP4K4-induced activation of JNK. These results suggest that MAP4K4 is a putative effector of Rap2 mediating the activation of JNK by Rap2.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
16
pubmed:volume
279
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
15711-4
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:14966141-Animals, pubmed-meshheading:14966141-Enzyme Activation, pubmed-meshheading:14966141-Humans, pubmed-meshheading:14966141-Intracellular Signaling Peptides and Proteins, pubmed-meshheading:14966141-JNK Mitogen-Activated Protein Kinases, pubmed-meshheading:14966141-Mice, pubmed-meshheading:14966141-Mitogen-Activated Protein Kinases, pubmed-meshheading:14966141-Molecular Sequence Data, pubmed-meshheading:14966141-Mutation, pubmed-meshheading:14966141-NIH 3T3 Cells, pubmed-meshheading:14966141-Protein Isoforms, pubmed-meshheading:14966141-Protein Structure, Tertiary, pubmed-meshheading:14966141-Protein-Serine-Threonine Kinases, pubmed-meshheading:14966141-Signal Transduction, pubmed-meshheading:14966141-Substrate Specificity, pubmed-meshheading:14966141-rap GTP-Binding Proteins
pubmed:year
2004
pubmed:articleTitle
Mitogen-activated protein kinase kinase kinase kinase 4 as a putative effector of Rap2 to activate the c-Jun N-terminal kinase.
pubmed:affiliation
Division of Cell Biology, Graduate School of Medicine, Faculty of Medicine, University of the Ryukyus, 207 Uehara, Nishihara-cho, Okinawa 903-0215, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't