Linked Life Data

14964778

Source:http://linkedlifedata.com/resource/pubmed/id/14964778

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2004-2-13
pubmed:abstractText
1. Sucrose feeding increases the level of stress-induced heat shock protein 70 mRNA in the rat hypothalamus. However, the mechanism by which a sucrose diet induces mRNA remains unclear. The issues investigated in this study were (1) whether a sucrose diet affects nitric oxide production in the hypothalamus, and (2) whether nitric oxide mediates the sucrose and stress-induced elevation of heat shock protein 70 mRNA. 2. To address the first question, we measured the level of nitrate, a final nitric-oxide-oxidation product measurable in vivo, using a microdialysis method. To address the second question, we administered a nitric oxide synthase inhibitor. NG-nitro-L-arginine methyl ester, prior to stress, then measured the mRNA level of heat shock protein 70 by the reverse transcription-competitive polymerase chain reaction method. 3. After the initiation of restraint stress, rats fed a sucrose-containing diet, unlike those fed standard chow, displayed a transient nitrate elevation. This nitrate elevation was attenuated by pretreatment with NG-nitro-L-arginine methyl ester. The mRNA level increases in rats fed a sucrose diet were dose-dependently attenuated by NG-nitro-L-arginine methyl ester. 4. These data suggest that a sucrose diet induces heat shock protein 70 under stress by enhancing nitric oxide production in the hypothalamus.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0272-4340
pubmed:author
pubmed:issnType
Print
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
907-15
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
2003
pubmed:articleTitle
Nitric oxide induced heat shock protein 70 mRNA in rat hypothalamus during acute restraint stress under sucrose diet.
pubmed:affiliation
Department of Psychiatry, Kitasato University School of Medicine, Sagamihara, Kanagawa, Japan. e-suzuki@kitasato-u.ac.jp
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't