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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5070
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pubmed:dateCreated |
1992-9-4
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pubmed:abstractText |
Serum and growth factors can increase the proportion of Ras in the active guanosine triphosphate (GTP)-bound form. Growth factors might stimulate guanine nucleotide exchange or decrease the activity of the guanosine triphosphatase-activating proteins GAP and neurofibromin (NF1). In NIH 3T3 cells that overexpress the mutant Ras protein His116, which releases bound guanine nucleotide at a constitutively high rate and retains sensitivity to GAP and NF1, the proportion of GTP bound to the His116 protein was not altered by serum or platelet-derived growth factor. However, these mitogens increased the proportion of Ras in the GTP-bound form in cells that overexpressed control Ras proteins with a normal intrinsic rate of guanine nucleotide release. The amount of GTP-bound His116 or control Ras proteins was higher in cells at low density than in cells at high density, which have more GAP-like activity. The lower proportion of GTP-bound Ras in NIH 3T3 cells at high density may result from increased GAP-like activity. By contrast, serum and platelet-derived growth factors appear to stimulate guanine nucleotide exchange.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0036-8075
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
31
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pubmed:volume |
257
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pubmed:geneSymbol |
c-ras
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
671-4
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pubmed:dateRevised |
2007-3-19
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pubmed:meshHeading |
pubmed-meshheading:1496380-3T3 Cells,
pubmed-meshheading:1496380-Animals,
pubmed-meshheading:1496380-Blood,
pubmed-meshheading:1496380-Cell Line, Transformed,
pubmed-meshheading:1496380-Fibroblasts,
pubmed-meshheading:1496380-GTPase-Activating Proteins,
pubmed-meshheading:1496380-Gene Expression,
pubmed-meshheading:1496380-Genes, ras,
pubmed-meshheading:1496380-Growth Substances,
pubmed-meshheading:1496380-Guanosine Triphosphate,
pubmed-meshheading:1496380-Histidine,
pubmed-meshheading:1496380-Methionine,
pubmed-meshheading:1496380-Mice,
pubmed-meshheading:1496380-Mutagenesis,
pubmed-meshheading:1496380-Neurofibromin 1,
pubmed-meshheading:1496380-Platelet-Derived Growth Factor,
pubmed-meshheading:1496380-Proteins,
pubmed-meshheading:1496380-Proto-Oncogene Proteins p21(ras),
pubmed-meshheading:1496380-Signal Transduction,
pubmed-meshheading:1496380-ras GTPase-Activating Proteins
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pubmed:year |
1992
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pubmed:articleTitle |
Mechanistic aspects of signaling through Ras in NIH 3T3 cells.
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pubmed:affiliation |
Laboratory of Cellular Oncology, National Cancer Institute, Bethesda, MD 20892.
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pubmed:publicationType |
Journal Article
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