14961371

Source:http://linkedlifedata.com/resource/pubmed/id/14961371

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0012854, umls-concept:C0185115, umls-concept:C0205352, umls-concept:C0442711, umls-concept:C0456962
pubmed:issue	2
pubmed:dateCreated	2004-2-12
pubmed:abstractText	DNA extraction of thraustochytrids, common marine unicellular organisms, is usually accomplished by either the cetyltrimethylammonium bromide (CTAB) or proteinase K protocols. A novel lysis buffer protocol for thraustochytrid total DNA extraction is described. The average isolated total DNA is 20 to 40 kb, and DNA samples are suitable for a variety of uses including 18S-ribosomal DNA polymerase chain reaction, restriction enzyme digestions, and amplified fragment length polymorphism analyses. The new protocol is also faster than the other protocols.
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/100892712
pubmed:status	PubMed-not-MEDLINE
pubmed:month	Mar
pubmed:issn	1436-2228
pubmed:author	pubmed-author:NiZZ, pubmed-author:RinkevichBB
pubmed:issnType	Print
pubmed:volume	3
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	100-2
pubmed:year	2001
pubmed:articleTitle	A simple, reliable, and fast protocol for thraustochytrid DNA extraction.
pubmed:affiliation	Minerva Center for Marine Invertebrate Immunology and Developmental Biology, Israel Oceanographic and Limnological Research, National Institute of Oceanography, P.O. Box 8030, Tel Shikmona, Haifa 31080, Israel.
pubmed:publicationType	Journal Article