Linked Life Data

14961315

Source:http://linkedlifedata.com/resource/pubmed/id/14961315

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Supplement 1
pubmed:dateCreated
2004-2-12
pubmed:abstractText
We adapted transgenic rodent mutation assays based on fish carrying bacteriophage lambda and plasmid pUR288 vectors to address the needs for improved methods to assess health risks from exposure to environmental mutagens and also to establish new animal models to study in vivo mutagenesis. The approach entails separating the vectors from fish genomic DNA and then shuttling them into specialized strains of E. coli bacteria to analyze spontaneous and induced mutations in either lacI and cII or lacZ mutational targets. Fish exhibited low frequencies of spontaneous mutants comparable to the sensitivity of transgenic rodent models. Mutations detected after treating fish with chemical mutagens showed concentration-dependent, tissue-specific, and time-dependent relationships. Spontaneous and induced mutational spectra also were consistent with the specificity of known mutagens, further supporting the utility of transgenic fish for studies of in vivo mutagenesis.
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
Jun
pubmed:issn
1436-2228
pubmed:author
pubmed:issnType
Print
pubmed:volume
3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
S185-95
pubmed:year
2001
pubmed:articleTitle
Bacteriophage lambda and plasmid pUR288 transgenic fish models for detecting in vivo mutations.
pubmed:affiliation
Aquatic Biotechnology and Environmental Laboratory, Warnell School of Forest Resources, University of Georgia, Athens, GA 30602, USA. rwinn@smokey.forestry.uga.edu
pubmed:publicationType
Journal Article