Source:http://linkedlifedata.com/resource/pubmed/id/14960622
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
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| pubmed:dateCreated |
2004-2-12
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| pubmed:abstractText |
The endoplasmic reticulum (ER) stress response contributes to neuronal survival in ischemia and neurodegenerative processes. ORP150 (oxygen-regulated protein 150)/HSP12A (heat shock protein 12A), a novel stress protein located in the ER, was markedly induced in Purkinje cells maximally at 4-8 d after birth, a developmental period corresponding to their vulnerability to cell death. Both terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end-labeling analysis and immunostaining using anti-activated caspase-3 antibody revealed that transgenic mice with targeted neuronal overexpression of ORP150 (Tg ORP150) displayed diminished cell death in the Purkinje cell layer and increased numbers of Purkinje cells up to 40 d after birth (p < 0.01), compared with those observed in heterozygous ORP150/HSP12A-deficient (ORP150+/-) mice and wild-type littermates (ORP150+/+). Cultured Purkinje cells from Tg ORP150 mice displayed resistance to both hypoxia- and AMPA-induced stress. Behavioral analysis, using rotor rod tasks, indicated impairment of cerebellar function in Tg ORP150 animals, consistent with the concept that enhanced survival of Purkinje cells results in dysfunction. These data suggest that ER chaperones have a pivotal role in Purkinje cell survival and death and thus may highlight the importance of ER stress in neuronal development.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Binding Protein, Vitamin...,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/HSP70 Heat-Shock Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/calbindin,
http://linkedlifedata.com/resource/pubmed/chemical/oxygen-regulated proteins
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| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
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| pubmed:issn |
1529-2401
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:day |
11
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| pubmed:volume |
24
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1486-96
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:14960622-Animals,
pubmed-meshheading:14960622-Animals, Newborn,
pubmed-meshheading:14960622-Behavior, Animal,
pubmed-meshheading:14960622-Brain,
pubmed-meshheading:14960622-Calcium-Binding Protein, Vitamin D-Dependent,
pubmed-meshheading:14960622-Calcium-Binding Proteins,
pubmed-meshheading:14960622-Cell Count,
pubmed-meshheading:14960622-Cell Death,
pubmed-meshheading:14960622-Cell Survival,
pubmed-meshheading:14960622-Cells, Cultured,
pubmed-meshheading:14960622-Cerebellum,
pubmed-meshheading:14960622-Endoplasmic Reticulum,
pubmed-meshheading:14960622-HSP70 Heat-Shock Proteins,
pubmed-meshheading:14960622-Heterozygote,
pubmed-meshheading:14960622-In Situ Nick-End Labeling,
pubmed-meshheading:14960622-Mice,
pubmed-meshheading:14960622-Mice, Inbred C57BL,
pubmed-meshheading:14960622-Mice, Mutant Strains,
pubmed-meshheading:14960622-Mice, Transgenic,
pubmed-meshheading:14960622-Patch-Clamp Techniques,
pubmed-meshheading:14960622-Proteins,
pubmed-meshheading:14960622-Purkinje Cells,
pubmed-meshheading:14960622-Stress, Physiological
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| pubmed:year |
2004
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| pubmed:articleTitle |
ORP150/HSP12A regulates Purkinje cell survival: a role for endoplasmic reticulum stress in cerebellar development.
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| pubmed:affiliation |
Department of Neuroanatomy, Graduate School of Medical Science, Kanazawa University, Kanazawa 920-8640, Japan. kitao@nanat.m.kanazawa-u.ac.jp
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| pubmed:publicationType |
Journal Article
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