Linked Life Data

14960085

Source:http://linkedlifedata.com/resource/pubmed/id/14960085

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2004-2-12
pubmed:abstractText
The effects of the nonlinear behavior of fluorescent intensity with excitation intensity on emission reabsorption laser-induced fluorescence (ERLIF) are investigated. Excitation nonlinearities arise mainly as a consequence of the depletion of the ground-state population stemming from the finite lifetime of molecules in the excited state. These nonlinearities hinder proper suppression of the excitation intensity information in the fluorescence ratio, degrading measurement accuracy. A method for minimizing this effect is presented. This method is based on the approximation of the fluorescence intensity nonlinearities by a power law. Elevating the two-dimensional fluorescent intensity maps to the appropriate exponent allows for proper suppression of excitation intensity in the fluorescence ratio. An overview of the principles and constitutive equations behind ERLIF film-thickness measurements, along with a characterization of the fluorescence's nonlinear behavior, is presented. The power law approximation and processing scheme used to mitigate this behavior are introduced. Experimental proof of the validity of the approximation and processing scheme is provided.
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
Feb
pubmed:issn
0003-6935
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
43
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
894-913
pubmed:year
2004
pubmed:articleTitle
Excitation nonlinearities in emission reabsorption laser-induced fluorescence techniques.
pubmed:affiliation
Department of Mechanical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, USA. hidrovo@stanford.edu
pubmed:publicationType
Journal Article