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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
15
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pubmed:dateCreated |
1992-9-4
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pubmed:abstractText |
We have developed a method of specifically modifying the mammalian genome in vivo. This procedure comprises heritable tissue-specific and site-specific DNA recombination as a function of recombinase expression in transgenic mice. Transgenes encoding the bacteriophage P1 Cre recombinase and the loxP-flanked beta-galactosidase gene were used to generate transgenic mice. Genomic DNA from doubly transgenic mice exhibited tissue-specific DNA recombination as a result of Cre expression. Further characterization revealed that this process was highly efficient at distinct chromosomal integration sites. These studies also imply that Cre-mediated recombination provides a heritable marker for mitoses following the loss of Cre expression. This transgene-recombination system permits unique approaches to in vivo studies of gene function within experimentally defined spatial and temporal boundaries.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1495975-1660141,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1495975-1708890,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1495975-1900642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1495975-1965144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1495975-2015626,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1495975-2035025,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1495975-2123451,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1495975-2288914,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1495975-2416464,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1495975-2495524,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1495975-2509077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1495975-2660260,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1495975-2686032,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1495975-2783482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1495975-2839833,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1495975-3011407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1495975-3037344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1495975-3071260,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1495975-3287623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1495975-518835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1495975-6220808,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1495975-6276557,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1495975-6319400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1495975-6400653
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD4,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD8,
http://linkedlifedata.com/resource/pubmed/chemical/Cre recombinase,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Nucleotidyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Probes,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Integrases,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Galactosidase
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
89
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pubmed:geneSymbol |
loxP
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6861-5
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1495975-Animals,
pubmed-meshheading:1495975-Antigens, CD4,
pubmed-meshheading:1495975-Antigens, CD8,
pubmed-meshheading:1495975-Blotting, Southern,
pubmed-meshheading:1495975-Coliphages,
pubmed-meshheading:1495975-DNA,
pubmed-meshheading:1495975-DNA Nucleotidyltransferases,
pubmed-meshheading:1495975-DNA Probes,
pubmed-meshheading:1495975-Growth Hormone,
pubmed-meshheading:1495975-Humans,
pubmed-meshheading:1495975-Integrases,
pubmed-meshheading:1495975-Mice,
pubmed-meshheading:1495975-Mice, Transgenic,
pubmed-meshheading:1495975-Organ Specificity,
pubmed-meshheading:1495975-Recombination, Genetic,
pubmed-meshheading:1495975-Restriction Mapping,
pubmed-meshheading:1495975-T-Lymphocytes,
pubmed-meshheading:1495975-Transcription, Genetic,
pubmed-meshheading:1495975-Viral Proteins,
pubmed-meshheading:1495975-beta-Galactosidase
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pubmed:year |
1992
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pubmed:articleTitle |
Tissue- and site-specific DNA recombination in transgenic mice.
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pubmed:affiliation |
Biomedical Research Centre, University of British Columbia, Vancouver, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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