Linked Life Data

14927802

Source:http://linkedlifedata.com/resource/pubmed/id/14927802

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1952-12-1
pubmed:abstractText
The calcification of rat hypertrophic cartilage slices in vitro is markedly inhibited by preliminary exposure to metachromatic and other basic dyes. The dyes are effective at 10(-3) to 10(-4)M in the absence of calcium and phosphate. This inhibition does not occur at the same low dye concentration if calcium and phosphate are present. Neither ion alone is effective in preventing the inhibition. The inhibitory action can be removed by placing slices which have been treated with basic dye in a solution which contains calcium and phosphate ions, plus an acid dye, Orange G. Most acid dyes do not inhibit calcification, except at very high concentrations. Alizarin and quinalizarin are exceptional, and produce marked inhibition at 10(-3)M, an effect which is perhaps attributable to the tendency of these dyes to form lakes with calcium. Rachitic cartilage slices which no longer calcify in inorganic phosphate as a result of treatment with basic dyes show normal calcification in the presence of organic phosphate esters. These results are discussed in terms of the possibility that chondroitin sulfate ester participates in normal calcification.
pubmed:commentsCorrections
pubmed:keyword
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
OM
pubmed:status
OLDMEDLINE
pubmed:month
May
pubmed:issn
0022-1007
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
95
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
497-508
pubmed:dateRevised
2010-6-22
pubmed:meshHeading
pubmed:year
1952
pubmed:articleTitle
The effect of dyes on the calcification of hypertrophic rachitic cartilage in vitro.
pubmed:publicationType
Journal Article