Linked Life Data

1490175

Source:http://linkedlifedata.com/resource/pubmed/id/1490175

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1993-2-26
pubmed:abstractText
By weighting the PCR reaction in favor of specificity for the target sequence in the beginning cycles and for continued efficient amplification of the sequence into later cycles, we were able to show an improvement in the specificity and quantity of amplified ras and p53 sequences. Increased purity and yield of specific products favorably enhanced post-PCR evaluation and interpretation of results using direct sequencing and single-stranded conformation polymorphism (SSCP) analysis when point mutations were present in DNA from tumor cell lines and tissues.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1054-9803
pubmed:author
pubmed:issnType
Print
pubmed:volume
2
pubmed:geneSymbol
H-ras, K-ras, N-ras, ras
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
45-50
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
PCR regimen for enhanced specificity and yield of targeted genomic DNA sequences: ras and p53.
pubmed:affiliation
Hematologic Diseases Branch, Centers for Disease Control, Atlanta, Georgia 30333.
pubmed:publicationType
Journal Article