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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
1993-2-23
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pubmed:abstractText |
One- to 16-cell porcine embryos were cultured in either Whittens medium supplemented with bovine serum albumin and fetal calf serum (WM) or in the same medium with porcine oviduct epithelial cell co-culture (WM-Poec). All stages of embryos cultured in WM-POEC had higher cell counts after 144-168 h of development than did embryos in WM. There was however, no significant difference in blastocyst formation rate of embryos cultured in WM-POEC over those cultured in WM. A high proportion of the embryos entering culture at the 1-2-cell were able to pass the 4-cell block stage in both WM and WM-POEC, 81% and 77%, respectively. In both media, most of the 1-2-cell embryos arrested their development at the compacted morula stage and failed to blastulate while embryos initiating culture at the 4- and 8-16-cell embryos formed blastocysts in culture at a rate of 80-90%.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0044-605X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
33
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
349-55
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1488950-Animals,
pubmed-meshheading:1488950-Blastocyst,
pubmed-meshheading:1488950-Cells, Cultured,
pubmed-meshheading:1488950-Culture Media,
pubmed-meshheading:1488950-Culture Techniques,
pubmed-meshheading:1488950-Epithelial Cells,
pubmed-meshheading:1488950-Fallopian Tubes,
pubmed-meshheading:1488950-Female,
pubmed-meshheading:1488950-Swine
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pubmed:year |
1992
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pubmed:articleTitle |
Oviduct epithelial cell co-culture of early porcine embryos.
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pubmed:affiliation |
Department of Clinical Studies, Reproduction, Royal Veterinary and Agricultural University Frederiksberg, Denmark.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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