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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2004-2-11
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pubmed:abstractText |
Melanoma antigen recognized by T cell 1 (MART-1) is regarded as a candidate peptide for vaccination against malignant melanoma, and it is of importance to develop strategies to improve the vaccine-elicited T-cell activation towards MART-1. T-cell activation is, among other determinants, dependent on the density of specific major histocompatibility complex-peptide complexes on the surface of the antigen-presenting cell. In this study, we explored the cell-surface presentation of a substituted MART-1 peptide encoded by transfected minigenes. We investigated the potential of proteasomal targeting compared to non-proteasomal targeting of the epitope to increase its cell-surface presentation. Furthermore, we explored the potential of incorporating multiple minigenes instead of one to increase cell-surface presentation. We show that both proteasomal targeting and repetition of the minigene increase cell-surface presentation of the epitope and propose both these approaches as potential strategies in DNA vaccines to increase MART-1-specific T-cell activation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Cancer Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/Cysteine Endopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/MART-1 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/MLANA protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Multienzyme Complexes,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proteasome Endopeptidase Complex,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, DNA
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0300-9475
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
59
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
220-7
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:14871300-Amino Acid Sequence,
pubmed-meshheading:14871300-Antigen Presentation,
pubmed-meshheading:14871300-Antigens, Neoplasm,
pubmed-meshheading:14871300-Blotting, Western,
pubmed-meshheading:14871300-Cancer Vaccines,
pubmed-meshheading:14871300-Cell Line,
pubmed-meshheading:14871300-Cysteine Endopeptidases,
pubmed-meshheading:14871300-Cytotoxicity Tests, Immunologic,
pubmed-meshheading:14871300-Epitopes,
pubmed-meshheading:14871300-Humans,
pubmed-meshheading:14871300-MART-1 Antigen,
pubmed-meshheading:14871300-Melanoma,
pubmed-meshheading:14871300-Molecular Sequence Data,
pubmed-meshheading:14871300-Multienzyme Complexes,
pubmed-meshheading:14871300-Neoplasm Proteins,
pubmed-meshheading:14871300-Plasmids,
pubmed-meshheading:14871300-Polymerase Chain Reaction,
pubmed-meshheading:14871300-Proteasome Endopeptidase Complex,
pubmed-meshheading:14871300-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:14871300-T-Lymphocytes,
pubmed-meshheading:14871300-Transfection,
pubmed-meshheading:14871300-Vaccines, DNA
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pubmed:year |
2004
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pubmed:articleTitle |
Proteasomal targeting and minigene repetition improve cell-surface presentation of a transfected, modified melanoma tumour antigen.
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pubmed:affiliation |
Institute of Medical Microbiology and Immunology, The Panum Institute, University of Copenhagen, Copenhagen, Denmark.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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