| pubmed-article:14871290 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:14871290 | lifeskim:mentions | umls-concept:C0020962 | lld:lifeskim |
| pubmed-article:14871290 | lifeskim:mentions | umls-concept:C0032136 | lld:lifeskim |
| pubmed-article:14871290 | lifeskim:mentions | umls-concept:C1515877 | lld:lifeskim |
| pubmed-article:14871290 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:14871290 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:14871290 | pubmed:dateCreated | 2004-2-11 | lld:pubmed |
| pubmed-article:14871290 | pubmed:abstractText | Bacterial DNA differs from mammalian DNA by the presence of unmethylated cytosine-phosphate-guanosine (CpG) motifs. The immunostimulatory properties of a DNA vaccine have been suspected to be associated with these motifs. The aim of this study was to assess the inactivation of the immunostimulatory potential of a plasmid after methylation of its CpG motifs. We constructed two identical non-coding plasmids, and one of these was de novo methylated on its CG sequences. A single administration of recombinant antigen with methylated or unmethylated CpG-containing plasmid was performed in mice. As expected, only unmethylated CpG-containing plasmid enhanced the specific immune response. However, a study of in vivo activation of Langerhans' cells and analysis of mRNA synthesis indicated that both the plasmids promoted cell emigration and cytokine induction. These data highlight that a methylated CpG-containing plasmid is not inert and carries immunomodulatory properties. The results further emphasize the necessity to definitively identify the mode of action of plasmids used for DNA vaccination. | lld:pubmed |
| pubmed-article:14871290 | pubmed:language | eng | lld:pubmed |
| pubmed-article:14871290 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14871290 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:14871290 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14871290 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14871290 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14871290 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14871290 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14871290 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14871290 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14871290 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:14871290 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:14871290 | pubmed:issn | 0300-9475 | lld:pubmed |
| pubmed-article:14871290 | pubmed:author | pubmed-author:CornélisAA | lld:pubmed |
| pubmed-article:14871290 | pubmed:author | pubmed-author:CapronMM | lld:pubmed |
| pubmed-article:14871290 | pubmed:author | pubmed-author:BayPP | lld:pubmed |
| pubmed-article:14871290 | pubmed:author | pubmed-author:LundCC | lld:pubmed |
| pubmed-article:14871290 | pubmed:author | pubmed-author:Poulain-Godef... | lld:pubmed |
| pubmed-article:14871290 | pubmed:author | pubmed-author:VendevilleCC | lld:pubmed |
| pubmed-article:14871290 | pubmed:author | pubmed-author:RiveauGG | lld:pubmed |
| pubmed-article:14871290 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:14871290 | pubmed:volume | 59 | lld:pubmed |
| pubmed-article:14871290 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:14871290 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:14871290 | pubmed:pagination | 143-51 | lld:pubmed |
| pubmed-article:14871290 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:14871290 | pubmed:meshHeading | pubmed-meshheading:14871290... | lld:pubmed |
| pubmed-article:14871290 | pubmed:meshHeading | pubmed-meshheading:14871290... | lld:pubmed |
| pubmed-article:14871290 | pubmed:meshHeading | pubmed-meshheading:14871290... | lld:pubmed |
| pubmed-article:14871290 | pubmed:meshHeading | pubmed-meshheading:14871290... | lld:pubmed |
| pubmed-article:14871290 | pubmed:meshHeading | pubmed-meshheading:14871290... | lld:pubmed |
| pubmed-article:14871290 | pubmed:meshHeading | pubmed-meshheading:14871290... | lld:pubmed |
| pubmed-article:14871290 | pubmed:meshHeading | pubmed-meshheading:14871290... | lld:pubmed |
| pubmed-article:14871290 | pubmed:meshHeading | pubmed-meshheading:14871290... | lld:pubmed |
| pubmed-article:14871290 | pubmed:meshHeading | pubmed-meshheading:14871290... | lld:pubmed |
| pubmed-article:14871290 | pubmed:meshHeading | pubmed-meshheading:14871290... | lld:pubmed |
| pubmed-article:14871290 | pubmed:meshHeading | pubmed-meshheading:14871290... | lld:pubmed |
| pubmed-article:14871290 | pubmed:meshHeading | pubmed-meshheading:14871290... | lld:pubmed |
| pubmed-article:14871290 | pubmed:meshHeading | pubmed-meshheading:14871290... | lld:pubmed |
| pubmed-article:14871290 | pubmed:meshHeading | pubmed-meshheading:14871290... | lld:pubmed |
| pubmed-article:14871290 | pubmed:meshHeading | pubmed-meshheading:14871290... | lld:pubmed |
| pubmed-article:14871290 | pubmed:meshHeading | pubmed-meshheading:14871290... | lld:pubmed |
| pubmed-article:14871290 | pubmed:meshHeading | pubmed-meshheading:14871290... | lld:pubmed |
| pubmed-article:14871290 | pubmed:meshHeading | pubmed-meshheading:14871290... | lld:pubmed |
| pubmed-article:14871290 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:14871290 | pubmed:articleTitle | Methylated CpG-containing plasmid activates the immune system. | lld:pubmed |
| pubmed-article:14871290 | pubmed:affiliation | INSERM U547, Pasteur Institut de Lille, 59019 Lille Cedex, France. | lld:pubmed |
| pubmed-article:14871290 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:14871290 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
