|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
2004-2-11
|
|
pubmed:abstractText |
Bacterial DNA differs from mammalian DNA by the presence of unmethylated cytosine-phosphate-guanosine (CpG) motifs. The immunostimulatory properties of a DNA vaccine have been suspected to be associated with these motifs. The aim of this study was to assess the inactivation of the immunostimulatory potential of a plasmid after methylation of its CpG motifs. We constructed two identical non-coding plasmids, and one of these was de novo methylated on its CG sequences. A single administration of recombinant antigen with methylated or unmethylated CpG-containing plasmid was performed in mice. As expected, only unmethylated CpG-containing plasmid enhanced the specific immune response. However, a study of in vivo activation of Langerhans' cells and analysis of mRNA synthesis indicated that both the plasmids promoted cell emigration and cytokine induction. These data highlight that a methylated CpG-containing plasmid is not inert and carries immunomodulatory properties. The results further emphasize the necessity to definitively identify the mode of action of plasmids used for DNA vaccination.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Feb
|
|
pubmed:issn |
0300-9475
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
59
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
143-51
|
|
pubmed:dateRevised |
2008-11-21
|
|
pubmed:meshHeading |
pubmed-meshheading:14871290-Animals,
pubmed-meshheading:14871290-Antibody Formation,
pubmed-meshheading:14871290-CpG Islands,
pubmed-meshheading:14871290-DNA,
pubmed-meshheading:14871290-DNA, Bacterial,
pubmed-meshheading:14871290-DNA Methylation,
pubmed-meshheading:14871290-Female,
pubmed-meshheading:14871290-Histocytochemistry,
pubmed-meshheading:14871290-Interferon-gamma,
pubmed-meshheading:14871290-Interleukin-12,
pubmed-meshheading:14871290-Interleukin-4,
pubmed-meshheading:14871290-Langerhans Cells,
pubmed-meshheading:14871290-Mice,
pubmed-meshheading:14871290-Mice, Inbred BALB C,
pubmed-meshheading:14871290-Plasmids,
pubmed-meshheading:14871290-Polymerase Chain Reaction,
pubmed-meshheading:14871290-Recombinant Proteins,
pubmed-meshheading:14871290-Vaccines, DNA
|
|
pubmed:year |
2004
|
|
pubmed:articleTitle |
Methylated CpG-containing plasmid activates the immune system.
|
|
pubmed:affiliation |
INSERM U547, Pasteur Institut de Lille, 59019 Lille Cedex, France.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
