Linked Life Data

1486242

Source:http://linkedlifedata.com/resource/pubmed/id/1486242

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1993-2-22
pubmed:abstractText
Several naturally occurring ribozymes have now been well characterized with respect to their in vivo and in vitro activities. Through detailed biochemical and genetic analyses, it has become possible to alter the substrate specificity of each ribozyme using simple Watson-Crick base pairing. Several laboratories, therefore, have designed ribozymes to cleave viral or other cellular transcripts in vitro with the hope of developing these molecules as antiviral or therapeutic agents. In addition to Watson-Crick base pairing, however, other factors such as protein or RNA tertiary interactions are involved in the ribozyme cleavage activity. Although several engineered ribozymes have been used successfully to reduce gene expression in vivo, it is difficult to determine whether gene expression has been reduced by the cleaving activity of the ribozyme or by its inherent antisense activity. In order to discriminate between these two activities and optimize potentially therapeutic ribozymes, it is imperative to develop in vivo assays in which the antisense activity of ribozymes is negligible.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1045-4403
pubmed:author
pubmed:issnType
Print
pubmed:volume
2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
331-57
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Biological and functional aspects of catalytic RNAs.
pubmed:affiliation
Department of Molecular Genetics, Beckman Research Institute, City of Hope, Duarte, California 91010.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Review