Linked Life Data

1482607

Source:http://linkedlifedata.com/resource/pubmed/id/1482607

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1993-2-17
pubmed:abstractText
Due to the extreme fragility of ultrathin frozen sections of brain tissue, the cryoultramicrotomy of non-embedded tissue has not been sufficiently used for immunocytochemical studies of the central nervous system. Sections are easily disrupted by the liquid surface tension when a droplet of sucrose is used (method by Tokuyasu, 1973) for their transfer on the grids. Use of silicotungstic acid (Tsuji, 1986) in place of sucrose improved the preservation of the ultrastructure but still could not resolve the difficulty. This report describes a new procedure for transferring dry ultrathin frozen sections by means of electrostatic attraction induced on the membrane covering the grids. Once attached electrostatically to the membrane, the sections were retained by van der Waals' forces. The dry ultrathin frozen sections obtained from both fresh and fixed brains displayed good preservation of their ultrastructures over a large surface. This new method which electrostatically transfers dry ultrathin frozen sections, avoiding the use of any liquid, is expected to serve for the immunocytochemical identification of neuronal cell bodies and terminals as well as their neurotransmitters and enzymes in both fresh and fixed brains.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0914-9465
pubmed:author
pubmed:issnType
Print
pubmed:volume
55
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
423-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Cryoultramicrotomy: electrostatic transfer of dry ultrathin frozen sections on grids applied to the central nervous system.
pubmed:affiliation
Département de Cytologie, Université Paris VI, France.
pubmed:publicationType
Journal Article, Comparative Study