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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1993-2-9
|
| pubmed:abstractText |
The basis for the hypersensitive response of glycogen phosphorylase to epinephrine stimulation was investigated in adult rat cardiomyocytes isolated from normal and alloxan-diabetic animals. To assess potential G-protein involvement in the response, normal and diabetic derived myocytes were incubated with either cholera or pertussis toxin prior to hormonal stimulation. Pretreatment of cardiomyocytes with cholera toxin resulted in a potentiated response to epinephrine stimulation whereas pertussis toxin did not affect the activation of this signaling pathway. To determine if the enhanced response of phosphorylase activation resulted from an alteration in adenylate cyclase activation, the cells were challenged with forskolin. After 3 hr in primary culture, diabetic cardiomyocytes exhibited a hypersensitive response to forskolin stimulation relative to normal cells. However, after 24 hr in culture, both normal and diabetic myocytes responded identically to forskolin challenge. The present data suggest that a cholera toxin sensitive G-protein mediates the hypersensitive response of glycogen phosphorylase to catecholamine stimulation in diabetic cardiomyocytes and this response which is present in alloxan-diabetic cells and is induced in vitro in normal cardiomyocytes is primarily due to a defect at a post-receptor site.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenylate Cyclase,
http://linkedlifedata.com/resource/pubmed/chemical/Adenylate Cyclase Toxin,
http://linkedlifedata.com/resource/pubmed/chemical/Cholera Toxin,
http://linkedlifedata.com/resource/pubmed/chemical/Epinephrine,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Pertussis Toxin,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphorylases,
http://linkedlifedata.com/resource/pubmed/chemical/Virulence Factors, Bordetella
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0300-8177
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
4
|
| pubmed:volume |
117
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
63-70
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:1480165-Adenylate Cyclase,
pubmed-meshheading:1480165-Adenylate Cyclase Toxin,
pubmed-meshheading:1480165-Animals,
pubmed-meshheading:1480165-Cells, Cultured,
pubmed-meshheading:1480165-Cholera Toxin,
pubmed-meshheading:1480165-Diabetes Mellitus, Experimental,
pubmed-meshheading:1480165-Enzyme Activation,
pubmed-meshheading:1480165-Epinephrine,
pubmed-meshheading:1480165-GTP-Binding Proteins,
pubmed-meshheading:1480165-Heart,
pubmed-meshheading:1480165-Male,
pubmed-meshheading:1480165-Myocardium,
pubmed-meshheading:1480165-Pertussis Toxin,
pubmed-meshheading:1480165-Phosphorylases,
pubmed-meshheading:1480165-Rats,
pubmed-meshheading:1480165-Rats, Sprague-Dawley,
pubmed-meshheading:1480165-Reference Values,
pubmed-meshheading:1480165-Signal Transduction,
pubmed-meshheading:1480165-Virulence Factors, Bordetella
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Post-receptor defect accounts for phosphorylase hypersensitivity in cultured diabetic cardiomyocytes.
|
| pubmed:affiliation |
Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School, Worcester 01655.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|