Linked Life Data

1480018

Source:http://linkedlifedata.com/resource/pubmed/id/1480018

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1993-2-5
pubmed:abstractText
Stromelysin and stromelysin 2, closely related members of the metalloproteinase gene family degrade many non-collagenous components of the extracellular matrix and may play a role in the activation of latent procollagenase. Because we use monolayer cultures of rabbit and human fibroblasts as model systems to study these enzymes, we compared their expression in fibroblasts from both species. Rabbit stromelysin purified from fibroblast culture medium often appears as a protein doublet, while human stromelysin is a single protein band. Hybrid selection with a cDNA clone for rabbit stromelysin and in vitro translation of mRNA from rabbit fibroblasts stimulated with phorbol myristate acetate (PMA) reveals two translation products, Mr54 and 56KD, as measured by SDS polyacrylamide gel electrophoresis. In vitro transcription and translation of a 1.8 kb cDNA for rabbit stromelysin gives a single protein product, preprostromelysin, MR 56KD. We do not yet know whether the rabbit doublet represents two distinct gene products or whether it results from posttranscriptional/posttranslational processing of a single transcript or protein. To study human stromelysin, we cloned a cDNA from a rheumatoid synovial cell cDNA library and we used it to isolate genes for stromelysin and a related gene, stromelysin-2. Both genes are contained on approximately 14 kilobase pairs of DNA. With an exon containing fragment of the human stromelysin-2 genomic clone as a specific probe in Northern blot analysis, we demonstrate the differential expression of stromelysin and stromelysin 2 in rheumatoid synovial cells, human foreskin fibroblasts, and rabbit synovial fibroblasts. Chimeric constructs containing 302 bp of the human stromelysin promoter DNA linked to the bacterial gene chloramphenicol acetyl transferase (CAT) can be induced by PMA, epidermal growth factor (EGF) and interleukin-1 beta (IL-1 beta). Since the genes for stromelysin and stromelysin 2 are so conserved and since mechanisms regulating their expression appear to be distinctive, identification of these mechanisms in both rabbits and humans will increase our understanding of the relative role of these enzymes in normal and disease processes.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0940-1199
pubmed:author
pubmed:issnType
Print
pubmed:volume
1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
165-75
pubmed:dateRevised
2008-2-26
pubmed:meshHeading
pubmed-meshheading:1480018-Animals, pubmed-meshheading:1480018-Arthritis, Rheumatoid, pubmed-meshheading:1480018-Base Sequence, pubmed-meshheading:1480018-Cells, Cultured, pubmed-meshheading:1480018-Consensus Sequence, pubmed-meshheading:1480018-Dexamethasone, pubmed-meshheading:1480018-Enzyme Induction, pubmed-meshheading:1480018-Enzyme Precursors, pubmed-meshheading:1480018-Fibroblasts, pubmed-meshheading:1480018-Glycoproteins, pubmed-meshheading:1480018-Humans, pubmed-meshheading:1480018-Infant, Newborn, pubmed-meshheading:1480018-Male, pubmed-meshheading:1480018-Matrix Metalloproteinase 10, pubmed-meshheading:1480018-Matrix Metalloproteinase 3, pubmed-meshheading:1480018-Metalloendopeptidases, pubmed-meshheading:1480018-Molecular Sequence Data, pubmed-meshheading:1480018-Penis, pubmed-meshheading:1480018-Proto-Oncogene Proteins c-jun, pubmed-meshheading:1480018-Rabbits, pubmed-meshheading:1480018-Recombinant Fusion Proteins, pubmed-meshheading:1480018-Sequence Homology, Nucleic Acid, pubmed-meshheading:1480018-Synovial Fluid, pubmed-meshheading:1480018-Tetradecanoylphorbol Acetate, pubmed-meshheading:1480018-Tretinoin
pubmed:year
1992
pubmed:articleTitle
Expression of stromelysin and stromelysin-2 in rabbit and human fibroblasts.
pubmed:affiliation
Department of Medicine, Dartmouth Medical School Hanover, NH 03756.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't