|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:dateCreated |
1993-2-8
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
We have identified a novel high molecular weight, vesicle-associated protein (VAP-1) in the eggs of the sea urchin Strongylocentrotus purpuratus. Biochemical fractionation and immunofluorescence analysis of unfertilized eggs indicate that VAP-1 is a peripheral membrane protein associated with microsomal membrane fractions. Sequence analysis of partial VAP-1 cDNA clones reveals that the protein contains at least four RNA-binding consensus sequences. The RNA-binding sequences are separated by several glycine rich domains and this organization, RNA-binding domains separated by glycine rich sequences, is common to several RNA-binding proteins including the heterogeneous ribonuclear protein A1 and nucleolin. The characteristics of VAP-1 suggest that the protein may function as a multidomain RNA-binding protein. The possibility that VAP-1 may play a role in nuclear RNA processing is also discussed.
|
|
pubmed:grant |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Nov
|
|
pubmed:issn |
0021-9533
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
103 ( Pt 3)
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
797-809
|
|
pubmed:dateRevised |
2007-11-14
|
|
pubmed:meshHeading |
pubmed-meshheading:1478972-Amino Acid Sequence,
pubmed-meshheading:1478972-Animals,
pubmed-meshheading:1478972-Base Sequence,
pubmed-meshheading:1478972-Consensus Sequence,
pubmed-meshheading:1478972-Egg Proteins,
pubmed-meshheading:1478972-Glycine,
pubmed-meshheading:1478972-Membrane Proteins,
pubmed-meshheading:1478972-Microsomes,
pubmed-meshheading:1478972-Molecular Sequence Data,
pubmed-meshheading:1478972-Ovum,
pubmed-meshheading:1478972-RNA-Binding Proteins,
pubmed-meshheading:1478972-Sea Urchins,
pubmed-meshheading:1478972-Sequence Alignment
|
|
pubmed:year |
1992
|
|
pubmed:articleTitle |
A novel vesicle-associated protein (VAP-1) in sea urchin eggs containing multiple RNA-binding consensus sequences.
|
|
pubmed:affiliation |
Department of Cell Biology and Anatomy, University of Miami School of Medicine, FL 33101.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
|
