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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1993-2-8
|
| pubmed:abstractText |
A streamlined version of direct dideoxy sequencing is presented that includes template preparation as well as sequencing protocols. The method is used routinely to sequence double-stranded PCR products after minimal purification with one of the primers used in amplification. Either 35S or 32P labeling can be used with equally good results.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
1054-9803
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
1
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
255-6
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:1477660-DNA-Directed DNA Polymerase,
pubmed-meshheading:1477660-Electrophoresis, Agar Gel,
pubmed-meshheading:1477660-Polymerase Chain Reaction,
pubmed-meshheading:1477660-Sequence Analysis, DNA,
pubmed-meshheading:1477660-Taq Polymerase,
pubmed-meshheading:1477660-Templates, Genetic
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Simplified template preparation and improved direct sequencing using Taq polymerase.
|
| pubmed:affiliation |
Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
|