| pubmed-article:14770006 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:14770006 | lifeskim:mentions | umls-concept:C0178539 | lld:lifeskim |
| pubmed-article:14770006 | lifeskim:mentions | umls-concept:C0035696 | lld:lifeskim |
| pubmed-article:14770006 | lifeskim:mentions | umls-concept:C2828366 | lld:lifeskim |
| pubmed-article:14770006 | lifeskim:mentions | umls-concept:C1705851 | lld:lifeskim |
| pubmed-article:14770006 | lifeskim:mentions | umls-concept:C1555707 | lld:lifeskim |
| pubmed-article:14770006 | lifeskim:mentions | umls-concept:C2752151 | lld:lifeskim |
| pubmed-article:14770006 | pubmed:dateCreated | 2004-2-10 | lld:pubmed |
| pubmed-article:14770006 | pubmed:abstractText | Removal of the 5' cap from a messenger RNA (mRNA) is an integral part of all mRNA decay pathways and can be a highly regulated event. Assays designed to assess decapping in vitro need to effectively resolve four products of mRNA decay: 7meGpppG produced by 3'-5' shortening of the transcript by the exosome, 7meGMP produced by the scavenger decapping enzyme DcpS acting on the product of exosomal decay, 7meGDP produced by the Dcp1/2 decapping enzyme, and free phosphate, which can be generated by phosphatases in the extract acting upon either of the two products of decapping noted above. We have outlined both thin-layer chromatography and acrylamide-gel based approaches that can be used to assess decapping activities. | lld:pubmed |
| pubmed-article:14770006 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14770006 | pubmed:language | eng | lld:pubmed |
| pubmed-article:14770006 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14770006 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:14770006 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14770006 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14770006 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14770006 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:14770006 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:14770006 | pubmed:issn | 1064-3745 | lld:pubmed |
| pubmed-article:14770006 | pubmed:author | pubmed-author:WiluszJeffrey... | lld:pubmed |
| pubmed-article:14770006 | pubmed:author | pubmed-author:BergmanNaomiN | lld:pubmed |
| pubmed-article:14770006 | pubmed:author | pubmed-author:OpyrchalMateu... | lld:pubmed |
| pubmed-article:14770006 | pubmed:author | pubmed-author:BatesElizabet... | lld:pubmed |
| pubmed-article:14770006 | pubmed:author | pubmed-author:MiloneJosephJ | lld:pubmed |
| pubmed-article:14770006 | pubmed:author | pubmed-author:BellofattoViv... | lld:pubmed |
| pubmed-article:14770006 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:14770006 | pubmed:volume | 257 | lld:pubmed |
| pubmed-article:14770006 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:14770006 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:14770006 | pubmed:pagination | 181-92 | lld:pubmed |
| pubmed-article:14770006 | pubmed:dateRevised | 2008-11-4 | lld:pubmed |
| pubmed-article:14770006 | pubmed:meshHeading | pubmed-meshheading:14770006... | lld:pubmed |
| pubmed-article:14770006 | pubmed:meshHeading | pubmed-meshheading:14770006... | lld:pubmed |
| pubmed-article:14770006 | pubmed:meshHeading | pubmed-meshheading:14770006... | lld:pubmed |
| pubmed-article:14770006 | pubmed:meshHeading | pubmed-meshheading:14770006... | lld:pubmed |
| pubmed-article:14770006 | pubmed:meshHeading | pubmed-meshheading:14770006... | lld:pubmed |
| pubmed-article:14770006 | pubmed:meshHeading | pubmed-meshheading:14770006... | lld:pubmed |
| pubmed-article:14770006 | pubmed:meshHeading | pubmed-meshheading:14770006... | lld:pubmed |
| pubmed-article:14770006 | pubmed:meshHeading | pubmed-meshheading:14770006... | lld:pubmed |
| pubmed-article:14770006 | pubmed:meshHeading | pubmed-meshheading:14770006... | lld:pubmed |
| pubmed-article:14770006 | pubmed:meshHeading | pubmed-meshheading:14770006... | lld:pubmed |
| pubmed-article:14770006 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:14770006 | pubmed:articleTitle | Assessing messenger RNA decapping in cellular extracts. | lld:pubmed |
| pubmed-article:14770006 | pubmed:affiliation | Department of Microbiology and Molecular Genetics, UMDNJ-New Jersey Medical School, Newark, NJ, USA. | lld:pubmed |
| pubmed-article:14770006 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:14770006 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
