Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2004-2-18
pubmed:abstractText
A 16-kDa plastid envelope protein was identified by chemical crosslinking that interacts with the precursor of NADPH:protochlorophyllide oxdidoreductase A (pPORA) during its posttranslational import into isolated barley chloroplasts. Protein purification and subsequent protein sequencing showed that the 16-kDa protein is an ortholog of a previously identified outer plastid envelope protein, Oep16. A protein of identical size was present in barley etioplasts and interacted with pPORA. Similar 16-kDa protein-dependent crosslink products of pPORA were detected in wheat, pea, and Arabidopsis chloroplasts. Database analyses revealed that the 16-kDa protein belongs to a family of preprotein and amino acid transporters found in free-living bacteria and endosymbiotic mitochondria and chloroplasts. Antibodies raised against the 16-kDa protein inhibited import of pPORA, highlighting its role in protein import.
pubmed:commentsCorrections
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pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
17
pubmed:volume
101
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2203-8
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:14769929-Amino Acid Sequence, pubmed-meshheading:14769929-Chloroplast Proteins, pubmed-meshheading:14769929-Cross-Linking Reagents, pubmed-meshheading:14769929-Evolution, Molecular, pubmed-meshheading:14769929-Intracellular Membranes, pubmed-meshheading:14769929-Ion Channels, pubmed-meshheading:14769929-Membrane Transport Proteins, pubmed-meshheading:14769929-Molecular Sequence Data, pubmed-meshheading:14769929-Molecular Weight, pubmed-meshheading:14769929-Oxidoreductases Acting on CH-CH Group Donors, pubmed-meshheading:14769929-Plant Cells, pubmed-meshheading:14769929-Plant Proteins, pubmed-meshheading:14769929-Plants, pubmed-meshheading:14769929-Plastids, pubmed-meshheading:14769929-Protein Binding, pubmed-meshheading:14769929-Protein Precursors, pubmed-meshheading:14769929-Protein Transport
pubmed:year
2004
pubmed:articleTitle
The outer plastid envelope protein Oep16: role as precursor translocase in import of protochlorophyllide oxidoreductase A.
pubmed:affiliation
Université Joseph Fourier et Centre National de la Recherche Scientifique, Unité Mixte de Recherche 5575, BP53, F-38041 Grenoble Cedex 9, France. steffen.reinbothe@ujf-grenoble.fr
pubmed:publicationType
Journal Article