Linked Life Data

1476795

Source:http://linkedlifedata.com/resource/pubmed/id/1476795

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1993-2-8
pubmed:abstractText
Yeast artificial chromosome (YAC) fragmentation vectors have proven to be useful reagents for mapping and modifying YAC clones through homologous recombination. Such vectors can be employed to remove a noncontiguous sequence present in chimeric YAC clones. However, previous fragmentation vectors have been based on auxotrophies not present in the background of most recombinant YAC libraries, which therefore makes them difficult to use. We have constructed an acentric YAC deletion vector pBCL that is fully compatible with AB 1380, the yeast host used in the majority of human total genomic YAC libraries. Transformation of three unrelated YACs with linearized pBCL yielded derivative clones with the expected phenotype at rates of 27%-49%. Analysis of a random cohort of these clones by pulsed-field gel electrophoresis and Southern blotting revealed single deleted structures in 31 of 34 clones. Using this vector, a nested set of deletion derivatives can efficiently and reliably be generated from human recombinant YAC clones.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1050-3862
pubmed:author
pubmed:issnType
Print
pubmed:volume
9
pubmed:geneSymbol
BCR, LYS2, TRP1, URA3
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
86-90
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Creation of a yeast artificial chromosome fragmentation vector based on lysine-2.
pubmed:affiliation
Department of Pediatrics, Gwynne Hazen Cherry Memorial Laboratories, Jonsson Comprehensive Cancer Center, School of Medicine, Los Angeles, California.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't