Source:http://linkedlifedata.com/resource/pubmed/id/14767819
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
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| pubmed:dateCreated |
2004-2-9
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| pubmed:abstractText |
During the past years, human coronaviruses (HCoVs) have been increasingly identified as pathogens associated with more-severe respiratory tract infection (RTI). Diagnostic tests for HCoVs are not frequently used in the routine setting. It is likely that, as a result, the precise role that HCoVs play in RTIs is greatly underestimated. We describe a rapid, sensitive, and highly specific quantitative real-time reverse-transcriptase polymerase chain reaction (RT-PCR) for the detection of HCoV that can easily be implemented in the routine diagnostic setting. HCoV was detected in 28 (11%) of the 261 clinical specimens obtained from patients presenting with symptoms of RTI ranging from common cold to severe pneumonia. Only 1 (0.4%) of the 243 control specimens obtained from patients without symptoms of RTI showed the presence of HCoV. We conclude that HCoVs can be frequently detected in patients presenting with RTI. Real-time RT-PCR provides a tool for large-scale epidemiological studies to further clarify the role that coronavirus infection plays in RTI in humans.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
|
| pubmed:issn |
0022-1899
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| pubmed:author |
pubmed-author:HendriksenKarin A WKA,
pubmed-author:HoepelmanAndy I MAI,
pubmed-author:NijhuisMoniqueM,
pubmed-author:OosterheertJan-JelrikJJ,
pubmed-author:SchipperPaulineP,
pubmed-author:SchuurmanRobR,
pubmed-author:van AlphenFlorisF,
pubmed-author:van EldenLeontine J RLJ,
pubmed-author:van KraaijMarian G JMG,
pubmed-author:van LoonAnton MAM
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| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
189
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
652-7
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| pubmed:dateRevised |
2004-11-17
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| pubmed:meshHeading |
pubmed-meshheading:14767819-Base Sequence,
pubmed-meshheading:14767819-Coronavirus,
pubmed-meshheading:14767819-Coronavirus 229E, Human,
pubmed-meshheading:14767819-Coronavirus Infections,
pubmed-meshheading:14767819-Coronavirus OC43, Human,
pubmed-meshheading:14767819-DNA Primers,
pubmed-meshheading:14767819-Humans,
pubmed-meshheading:14767819-Pneumonia,
pubmed-meshheading:14767819-Polymerase Chain Reaction,
pubmed-meshheading:14767819-Reference Values,
pubmed-meshheading:14767819-Respiratory Tract Infections
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| pubmed:year |
2004
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| pubmed:articleTitle |
Frequent detection of human coronaviruses in clinical specimens from patients with respiratory tract infection by use of a novel real-time reverse-transcriptase polymerase chain reaction.
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| pubmed:affiliation |
Department of Virology, Division of Acute Medicine and Infectious Diseases, University Medical Center Utrecht, PO Box 85500, 3508 GA Utrecht, The Netherlands. l.vanelden@azu.nl
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| pubmed:publicationType |
Journal Article
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