Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2004-3-24
pubmed:abstractText
In late mitosis and G1, a complex of the essential initiation proteins Mcm2-7 are assembled onto replication origins to 'license' them for initiation. At other times licensing is inhibited by cyclin-dependent kinases (CDKs) and geminin, thus ensuring that origins fire only once per cell cycle. Here we show that, paradoxically, CDKs are also required to inactivate geminin and activate the licensing system. On exit from metaphase in Xenopus laevis egg extracts, CDK-dependent activation of the anaphase-promoting complex (APC/C) results in the transient polyubiquitination of geminin. This ubiquitination triggers geminin inactivation without requiring ubiquitin-dependent proteolysis, and is essential for replication origins to become licensed. This reveals an unexpected role for CDKs and ubiquitination in activating chromosomal DNA replication.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-10465783, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-10606645, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-10644704, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-10678171, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-10766247, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-10766248, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-10995390, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-11125146, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-11146630, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-11175741, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-11429609, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-11432816, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-11849970, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-12045216, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-12049731, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-12059957, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-12080347, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-12651887, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-12713902, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-12860974, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-12915114, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-1992340, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-2203536, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-2530237, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-3357511, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-5106340, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-7758114, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-7760937, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-7760938, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-7925396, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-8087848, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-8253833, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-8354699, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-8407956, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-8799842, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-9008708, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-9030781, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-9251047, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-9456309, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-9566895, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-9635433, http://linkedlifedata.com/resource/pubmed/commentcorrection/14767479-9889196
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1465-7392
pubmed:author
pubmed:issnType
Print
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
260-7
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Non-proteolytic inactivation of geminin requires CDK-dependent ubiquitination.
pubmed:affiliation
Wellcome Trust Biocentre, University of Dundee, Dow Street, Dundee DD1 5EH, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't