Linked Life Data

1476659

Source:http://linkedlifedata.com/resource/pubmed/id/1476659

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1993-2-11
pubmed:abstractText
We applied a recently described technique which is based on a light transmission/scattering method to determine the association characteristics of the Geodia lectin to sheep erythrocytes. The agglutination assays were performed in a total volume of 3 ml with 5.4 x 10(6) erythrocytes/ml. At a concentration of 360 ng/ml 50% of the lectin molecules were bound to the cells within the first 10 s of incubation. Scatchard analyses revealed an association constant (K(a)) of 0.9 +/- 0.1 x 10(8) M-1 and a number of 3.8 +/- 0.6 x 10(6) lectin binding sites on one erythrocyte. The method was also successfully applied to determine quantitatively the inhibitory potential of sugars competing with cell surface glycoproteins for the lectin binding site. At a lectin concentration of 360 ng/ml 1 mM of lactose or 70 mM of D-N-acetylgalactosamine are required to inhibit lectin-mediated agglutination by 50%. These analyses confirm the potential of the novel light absorption agglutination technique to evaluate lectin characteristics.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0177-3593
pubmed:author
pubmed:issnType
Print
pubmed:volume
373
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1105-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Determination of lectin-cell-binding parameters by a new agglutination technique.
pubmed:affiliation
Abteilung Angewandte Molekularbiologie, Universität Mainz.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't