Linked Life Data

14764627

Source:http://linkedlifedata.com/resource/pubmed/id/14764627

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2004-4-16
pubmed:abstractText
Cocaine- and amphetamine-regulated transcript (CART) mRNA and CART peptide are abundant in the hypothalamic nuclei that control anterior pituitary function. CART peptide has also been localized in the anterior pituitary gland itself, although its role in pituitary function has not as yet been elucidated. In the present study, we investigated the localization and function of CART peptide in the anterior pituitary gland. Immunohistochemical observations revealed that CART peptide colocalized with FSH and LH in gonadotroph cells but that it was absent from the other hormone-producing cells. Immunoelectronmicroscopy suggested that CART peptide and gonadotropin were colocalized in the same secretory granules. CART peptide suppressed prolactin release from dispersed anterior pituitary cells 15 min after its addition into the media [basal production, 234.9 +/- 14.6 ng/ml vs. CART 55-102 peptide 100 nm, 125.0 +/- 18.2 ng/ml (P < 0.01, n = 5)]. Prolactin release was suppressed by CART in a dose-related manner; on the other hand, CART peptide did not affect the secretion of other anterior pituitary hormones. CART peptide synthesis by these cells was elevated 15 min after the addition of leptin to the media (100 nm), as determined by immunoblotting, but LHRH (10 nm) did not significantly affect CART peptide expression. These findings suggest that CART synthesis in the anterior pituitary may be stimulated by leptin and that CART peptide may play a role in the regulation of anterior pituitary hormone secretion in the rat.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0013-7227
pubmed:author
pubmed:issnType
Print
pubmed:volume
145
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2542-50
pubmed:dateRevised
2009-11-3
pubmed:meshHeading
pubmed-meshheading:14764627-Animals, pubmed-meshheading:14764627-Follicle Stimulating Hormone, pubmed-meshheading:14764627-Gene Expression, pubmed-meshheading:14764627-Gonadotropin-Releasing Hormone, pubmed-meshheading:14764627-Gonadotropins, pubmed-meshheading:14764627-Immunoblotting, pubmed-meshheading:14764627-Immunohistochemistry, pubmed-meshheading:14764627-Leptin, pubmed-meshheading:14764627-Luteinizing Hormone, pubmed-meshheading:14764627-Male, pubmed-meshheading:14764627-Microscopy, Immunoelectron, pubmed-meshheading:14764627-Nerve Tissue Proteins, pubmed-meshheading:14764627-Pituitary Gland, pubmed-meshheading:14764627-Prolactin, pubmed-meshheading:14764627-RNA, Messenger, pubmed-meshheading:14764627-Rats, pubmed-meshheading:14764627-Rats, Wistar, pubmed-meshheading:14764627-Secretory Vesicles
pubmed:year
2004
pubmed:articleTitle
Cocaine- and amphetamine-regulated transcript peptide in the rat anterior pituitary gland is localized in gonadotrophs and suppresses prolactin secretion.
pubmed:affiliation
Department of Pathology, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa 259-1193, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't