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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2004-5-10
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pubmed:abstractText |
Gastrin is a known growth/differentiation factor for the gastric mucosa. Its effects are likely mediated by the induction of heparin-binding epidermal-like growth factor (HB-EGF), a member of the EGF family of growth factors that is expressed by gastric parietal cells. In this study, we investigated the regulation of the HB-EGF promoter by gastrin in a human gastric cancer cell line. Serial human HB-EGF promoter-luciferase reporter deletion constructs and heterologous promoter constructs were transfected into AGS-E cells and stimulated with gastrin (10(-7) M) with or without various signal transduction inhibitors. EMSA were also performed. Gastrin stimulation resulted in a fivefold increase in HB-EGF-luciferase activity. The cis-acting element mediating gastrin responsiveness was mapped to the -69 to -58 region of the HB-EGF promoter. Gastrin stimulation was PKC dependent and at least partially mediated by activation of the EGF receptor.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0193-1857
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
286
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
G992-9
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:14764442-Animals,
pubmed-meshheading:14764442-Cell Line, Tumor,
pubmed-meshheading:14764442-Cell Nucleus,
pubmed-meshheading:14764442-Epidermal Growth Factor,
pubmed-meshheading:14764442-Gastrins,
pubmed-meshheading:14764442-Humans,
pubmed-meshheading:14764442-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:14764442-Mice,
pubmed-meshheading:14764442-Promoter Regions, Genetic,
pubmed-meshheading:14764442-Protein Kinase C,
pubmed-meshheading:14764442-Receptor, Epidermal Growth Factor,
pubmed-meshheading:14764442-Response Elements,
pubmed-meshheading:14764442-Signal Transduction,
pubmed-meshheading:14764442-Sp1 Transcription Factor,
pubmed-meshheading:14764442-Stereoisomerism,
pubmed-meshheading:14764442-Transcription Factors,
pubmed-meshheading:14764442-Ultraviolet Rays,
pubmed-meshheading:14764442-Zinc
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pubmed:year |
2004
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pubmed:articleTitle |
Gastrin regulates the heparin-binding epidermal-like growth factor promoter via a PKC/EGFR-dependent mechanism.
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pubmed:affiliation |
University of Massachusetts Memorial Medical Center, Division of Digestive Diseases and Nutrition, Worcester, 01655, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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