Linked Life Data

14759075

Source:http://linkedlifedata.com/resource/pubmed/id/14759075

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2004-2-4
pubmed:abstractText
The aim of this study was to investigate the regional expression of thyroid hormone nuclear receptor forms (TR(alpha) and TR(beta)) and isoform (TR(alpha1) and TR(beta2)) mRNAs in normal and neoplastic (benignant and malignant) human thyroid tissue. Tumor specimens from patients with thyroid carcinomas (papillary: 5 cases; follicular: 5 cases; anaplastic: 2 cases), thyroid follicular adenomas (7 cases) and tissue from normal thyroid glands (12 cases) were analyzed by in situ hybridization and semiquantitative RT-PCR for the expression of TR(alpha1) and beta, as well as for the isoform alpha2 that does not bind the hormone. In normal tissues, TR(alpha2) was expressed at lower levels compared to TR(alpha1) (alpha1/alpha2 = 4.3). In papillary and follicular carcinomas, the expression of TR(alpha1) and TR(beta) did not change as compared with normal thyroid tissue and adenomas (0.87 +/- 0.15 SD vs 0.89 +/- 0.17 densitometric units, DU, and 0.15 +/- 0.02 vs 0.14 +/- 0.03 DU, respectively). However, the expression of TR(alpha2) was significantly higher in differentiated carcinomas compared to normal thyroid tissue and adenomas (0.47 +/- 0.05 vs 0.20 +/- 0.05 DU, p < 0.05) with alpha1/alpha2 = 1.4. In anaplastic carcinoma all TRs were absent. We concluded that both normal and pathological thyroid tissues, with the exception of anaplastic carcinoma, express all TRs in thyreocites and that differentiated thyroid carcinomas are associated in enhancing the expression of TR(alpha2) mRNA.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0391-4097
pubmed:author
pubmed:issnType
Print
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1008-12
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:14759075-Adenocarcinoma, Follicular, pubmed-meshheading:14759075-Adult, pubmed-meshheading:14759075-Blotting, Northern, pubmed-meshheading:14759075-Carcinoma, pubmed-meshheading:14759075-Carcinoma, Papillary, pubmed-meshheading:14759075-Female, pubmed-meshheading:14759075-Gene Expression Regulation, Neoplastic, pubmed-meshheading:14759075-Humans, pubmed-meshheading:14759075-In Situ Hybridization, pubmed-meshheading:14759075-RNA, Neoplasm, pubmed-meshheading:14759075-Receptors, Thyroid Hormone, pubmed-meshheading:14759075-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:14759075-Thyroid Gland, pubmed-meshheading:14759075-Thyroid Hormone Receptors alpha, pubmed-meshheading:14759075-Thyroid Hormone Receptors beta, pubmed-meshheading:14759075-Thyroid Neoplasms
pubmed:year
2003
pubmed:articleTitle
Localization and expression of thyroid hormone receptors normal and neoplastic human thyroid.
pubmed:affiliation
Institute Regina Apostolorum, Albano, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't