Linked Life Data

14752288

Source:http://linkedlifedata.com/resource/pubmed/id/14752288

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2004-1-30
pubmed:abstractText
Acute phase proteins such as serum amyloid A proteins (SAAs) and serum amyloid P component (SAP) are induced in the liver after various insults (e.g., infection, injury). The cellular and molecular mechanisms controlling the expression of these acute phase proteins may be specifically designed for different insults. The roles of two central molecules of the lipopolysaccharide (LPS)-mediated inflammation pathway (CD14 and toll-like receptor 4 [Tlr4]) were investigated for the regulation of SAAs and SAP in the liver of mice after an 18% total body surface area burn injury. RT-PCR analysis revealed a subtype- and time-dependent induction of SAA mRNAs between 3 h and 3 days, while there was a peak induction of SAP mRNA at day 1. Marked elevations of SAA and SAP protein levels at day 1 supported the mRNA data. Furthermore, a differential regulation of SAAs and SAP mRNAs was noted between CD14 knockout (KO) and their control mice after injury. SAA protein was induced to a lesser degree after injury in C3H/HeJ (Tlr4-defective) mice than in their control mice. In addition, in both CD14 KO and C3H/HeJ mice, the induction of SAP protein was significantly reduced compared with respective controls. These data provide evidence that CD14 and Tlr4 participate, at least in part, in a cascade of signaling events that control the immediate-early and differential induction of SAAs and SAP in the liver after injury. They also suggest that LPS may be one of the initial inducing agents associated with these acute phase responses in the liver after injury.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1073-2322
pubmed:author
pubmed:issnType
Print
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
144-50
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:14752288-Acute-Phase Reaction, pubmed-meshheading:14752288-Animals, pubmed-meshheading:14752288-Antigens, CD14, pubmed-meshheading:14752288-Blotting, Western, pubmed-meshheading:14752288-Burns, pubmed-meshheading:14752288-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:14752288-Female, pubmed-meshheading:14752288-Lipopolysaccharides, pubmed-meshheading:14752288-Liver, pubmed-meshheading:14752288-Membrane Glycoproteins, pubmed-meshheading:14752288-Mice, pubmed-meshheading:14752288-Mice, Inbred C3H, pubmed-meshheading:14752288-Mice, Inbred C57BL, pubmed-meshheading:14752288-Mice, Knockout, pubmed-meshheading:14752288-RNA, Messenger, pubmed-meshheading:14752288-Receptors, Cell Surface, pubmed-meshheading:14752288-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:14752288-Serum Amyloid A Protein, pubmed-meshheading:14752288-Serum Amyloid P-Component, pubmed-meshheading:14752288-Time Factors, pubmed-meshheading:14752288-Toll-Like Receptor 4, pubmed-meshheading:14752288-Toll-Like Receptors
pubmed:year
2004
pubmed:articleTitle
Involvement of CD14 and toll-like receptor 4 in the acute phase response of serum amyloid A proteins and serum amyloid P component in the liver after burn injury.
pubmed:affiliation
Burn Research, Shriners Hospitals for Children Northern California, Department of Surgery, University of California at Davis, Sacramento 95817, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't