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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1993-2-1
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pubmed:abstractText |
We have used new specific primers and probe in a polymerase chain reaction (PCR) followed by Southern blot assays to detect Pneumocystis carinii in human bronchoalveolar lavage samples obtained from HIV-infected patients with pulmonary symptoms. To facilitate the procedure we developed a filtration technique without DNA extraction yielding a high sensitivity (18/18 positive results). The high specificity of the technique was shown by testing immunosuppressed patients without P. carinii pneumonia.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0890-8508
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
6
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
361-5
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1474976-Animals,
pubmed-meshheading:1474976-Base Sequence,
pubmed-meshheading:1474976-Blotting, Southern,
pubmed-meshheading:1474976-Bronchoalveolar Lavage Fluid,
pubmed-meshheading:1474976-DNA, Fungal,
pubmed-meshheading:1474976-DNA, Ribosomal,
pubmed-meshheading:1474976-HIV Infections,
pubmed-meshheading:1474976-Humans,
pubmed-meshheading:1474976-Molecular Sequence Data,
pubmed-meshheading:1474976-Oligodeoxyribonucleotides,
pubmed-meshheading:1474976-Oligonucleotide Probes,
pubmed-meshheading:1474976-Pneumocystis,
pubmed-meshheading:1474976-Pneumonia, Pneumocystis,
pubmed-meshheading:1474976-Polymerase Chain Reaction,
pubmed-meshheading:1474976-RNA, Ribosomal, 16S,
pubmed-meshheading:1474976-Rats,
pubmed-meshheading:1474976-Sequence Alignment,
pubmed-meshheading:1474976-Sequence Homology, Nucleic Acid,
pubmed-meshheading:1474976-Species Specificity,
pubmed-meshheading:1474976-Templates, Genetic
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pubmed:year |
1992
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pubmed:articleTitle |
An alternative to DNA extraction for the diagnosis of Pneumocystis carinii pneumonia by polymerase chain reaction using a new oligonucleotide probe.
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pubmed:affiliation |
INSERM U.13, Hôpital Claude Bernard, Paris, France.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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