Source:http://linkedlifedata.com/resource/pubmed/id/14749183
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
2004-1-29
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pubmed:abstractText |
The promotion of membrane fusion by the fusion (F) protein of human parainfluenza virus 3 (hPIV3) is dependent on a virus-specific contribution from the hemagglutinin-neuraminidase (HN) protein. By evaluation of chimeric hPIV3-Newcastle disease virus (NDV) HN proteins, we have previously shown that hPIV3-F-specificity is determined by a domain that extends from the middle of the membrane anchor to the 82nd residue in the ectodomain [Virology 209, (1995) 457; Arch. Virol. 13 (1997) 115]. If the corresponding NDV-derived residues replace the two C-terminal residues in this domain, no fusion is detected. However, these substitutions restore a glycosylation site present in NDV HN, but not in hPIV3 HN. Deletion of this site from a nested set of chimeras with hPIV3-derived N-terminal portions of decreasing length partially restores fusion, suggesting that an oligosaccharide near the top of hPIV3 HN stalk modulates fusion. In addition, further mutational analyses show that a chimera with only 125 N-terminal hPIV3-derived residues (72 in the stalk) actually promotes fusion more efficiently than the wt protein. These findings localize the C-terminus of the F-specific domain in hPIV3 HN a full 10 residues closer to the membrane than previously shown.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/F protein, parainfluenza virus 3,
http://linkedlifedata.com/resource/pubmed/chemical/HN Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Oligosaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0168-1702
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
99
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
177-85
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:14749183-Animals,
pubmed-meshheading:14749183-Cell Line,
pubmed-meshheading:14749183-Cricetinae,
pubmed-meshheading:14749183-Glycosylation,
pubmed-meshheading:14749183-HN Protein,
pubmed-meshheading:14749183-Membrane Fusion,
pubmed-meshheading:14749183-Newcastle disease virus,
pubmed-meshheading:14749183-Oligosaccharides,
pubmed-meshheading:14749183-Parainfluenza Virus 3, Human,
pubmed-meshheading:14749183-Protein Binding,
pubmed-meshheading:14749183-Protein Structure, Tertiary,
pubmed-meshheading:14749183-Recombinant Proteins,
pubmed-meshheading:14749183-Sequence Deletion,
pubmed-meshheading:14749183-Viral Fusion Proteins,
pubmed-meshheading:14749183-Viral Proteins
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pubmed:year |
2004
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pubmed:articleTitle |
An oligosaccharide at the C-terminus of the F-specific domain in the stalk of the human parainfluenza virus 3 hemagglutinin-neuraminidase modulates fusion.
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pubmed:affiliation |
Department of Molecular Genetics and Microbiology, University of Massachusetts, 55 Lake Avenue North, 0165-0122, Worcester, MA, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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