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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2004-1-29
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pubmed:abstractText |
To investigate the involvement of the mitogen-activated protein kinase (MAPK) family of extracellular signal-regulated kinase (ERK) 1 and 2 (MAPKerk1/2) in the vasopressin-mediated vasoconstriction in the rat aorta. Vasopressin-induced vasoconstriction was measured in isolated rat thoracic aortae in the presence or absence of MAPKerk1/2 kinase (MKKmek1/2) inhibitors. Thereafter the MAPKerk1/2 phosphorylation in the rat aorta was quantified using Western blot analysis. Vasopressin (1-300 nm) induced a concentration-dependent vasoconstriction, which could be inhibited concentration dependently by the selective MKKmek1/2 inhibitors, PD 98059 (10 and 100 microm) and U 0126 (10 and 100 microm). Western blot analysis revealed a 2.7 +/- 0.6-fold increase in the MAPKerk1/2 phosphorylation induced by vasopressin (300 nm). This phosphorylation could be dose dependently prevented by both PD 98059 (100 microm) and U 0126 (10 and 100 microm). These results indicate that vasoconstriction induced by vasopressin is partly regulated by the MAPKerk1/2 pathway.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Butadienes,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Flavonoids,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Nitriles,
http://linkedlifedata.com/resource/pubmed/chemical/PD 98059,
http://linkedlifedata.com/resource/pubmed/chemical/Potassium,
http://linkedlifedata.com/resource/pubmed/chemical/U 0126,
http://linkedlifedata.com/resource/pubmed/chemical/Vasoconstrictor Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Vasopressins
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0767-3981
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
18
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
45-50
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:14748753-Animals,
pubmed-meshheading:14748753-Aorta, Thoracic,
pubmed-meshheading:14748753-Blotting, Western,
pubmed-meshheading:14748753-Butadienes,
pubmed-meshheading:14748753-Enzyme Inhibitors,
pubmed-meshheading:14748753-Flavonoids,
pubmed-meshheading:14748753-Male,
pubmed-meshheading:14748753-Mitogen-Activated Protein Kinase 1,
pubmed-meshheading:14748753-Mitogen-Activated Protein Kinase 3,
pubmed-meshheading:14748753-Mitogen-Activated Protein Kinases,
pubmed-meshheading:14748753-Muscle, Smooth, Vascular,
pubmed-meshheading:14748753-Muscle Contraction,
pubmed-meshheading:14748753-Myocardial Contraction,
pubmed-meshheading:14748753-Nitriles,
pubmed-meshheading:14748753-Phosphorylation,
pubmed-meshheading:14748753-Potassium,
pubmed-meshheading:14748753-Rats,
pubmed-meshheading:14748753-Rats, Wistar,
pubmed-meshheading:14748753-Signal Transduction,
pubmed-meshheading:14748753-Vasoconstrictor Agents,
pubmed-meshheading:14748753-Vasopressins
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pubmed:year |
2004
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pubmed:articleTitle |
Vasopressin-induced vasoconstriction is dependent on MAPKerk1/2 phosphorylation.
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pubmed:affiliation |
The Department of Pharmacotherapy, Academic Medical Centre, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands. j.o.streefkerk@amc.uva.nl
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pubmed:publicationType |
Journal Article,
In Vitro
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