Linked Life Data

14747914

Source:http://linkedlifedata.com/resource/pubmed/id/14747914

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2004-1-28
pubmed:abstractText
Three-dimensional arrangement and subsequent transplantation of chondrocytic cells in resorbable polymers has been shown to be a promising technique for the treatment of cartilaginous defects. Engineering of artificial cartilage tissue includes dedifferentiation of chondrocytes in monolayer culture, the use of biodegradable matrices and polymer scaffolds, and re-expression of chondrocytic marker genes in three-dimensional culture. The aim of this study was to characterize molecularly the phenotypic changes occurring with autologous cartilage tissue engineering. Human articular chondrocytes were isolated, cultured in medium containing human serum, and expanded up to passage 3. Chondrocytes were embedded in human fibrinogen and in polyglactin-polydioxanon fleeces and cultured three-dimensionally up to 4 weeks. Dedifferentiation of chondrocytes in monolayers and formation of cartilage tissue in vitro or after subcutaneous transplantation into nude mice was assessed by gene expression analysis of typical chondrocytic genes, histology, and immunohistochemistry. The expansion of chondrocytes with human serum resulted in the induction of type I and type III collagens, whereas cartilage-specific type II collagen, cartilage oligomeric matrix protein, cartilage link protein, and aggrecan were repressed and induced again after three-dimensional arrangement of chondrocytes in polyglactin-polydioxanon. Transplantation experiments documented the synthesis of proteoglycan and cartilage-specific type II collagen in vivo. Three-dimensional arrangement of human articular chondrocytes in resorbable polyglactin-polydioxanon fleeces supports chondrogenic differentiation and the formation of a hyaline-like cartilaginous matrix in vitro and in vivo.
pubmed:language
ger
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0085-4530
pubmed:author
pubmed:issnType
Print
pubmed:volume
33
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
76-85
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:14747914-Aged, pubmed-meshheading:14747914-Animals, pubmed-meshheading:14747914-Biocompatible Materials, pubmed-meshheading:14747914-Biodegradation, Environmental, pubmed-meshheading:14747914-Cartilage, Articular, pubmed-meshheading:14747914-Cells, Cultured, pubmed-meshheading:14747914-Chondrocytes, pubmed-meshheading:14747914-Collagen Type I, pubmed-meshheading:14747914-Collagen Type II, pubmed-meshheading:14747914-Collagen Type III, pubmed-meshheading:14747914-Culture Media, pubmed-meshheading:14747914-Extracellular Matrix Proteins, pubmed-meshheading:14747914-Fibrinogen, pubmed-meshheading:14747914-Gene Expression, pubmed-meshheading:14747914-Humans, pubmed-meshheading:14747914-Immunohistochemistry, pubmed-meshheading:14747914-Mice, pubmed-meshheading:14747914-Mice, Nude, pubmed-meshheading:14747914-Middle Aged, pubmed-meshheading:14747914-Phenotype, pubmed-meshheading:14747914-Polydioxanone, pubmed-meshheading:14747914-Polyglactin 910, pubmed-meshheading:14747914-Polymers, pubmed-meshheading:14747914-Proteoglycans, pubmed-meshheading:14747914-Time Factors, pubmed-meshheading:14747914-Tissue Engineering, pubmed-meshheading:14747914-Transplantation, Heterologous
pubmed:year
2004
pubmed:articleTitle
[Molecular characterization of tissue-engineered articular chondrocyte transplants based on resorbable polymer fleece].
pubmed:affiliation
Labor für Tissue Engineering, Experimentelle Rheumatologie und klinische Immunologie, Medizinische Fakultät, Humboldt-Universität, Charité, Berlin. christian.kaps@charite.de
pubmed:publicationType
Journal Article, Comparative Study, English Abstract