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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2004-1-28
pubmed:databankReference
pubmed:abstractText
The characterization of restrictions to lentivirus replication in cells identifies critical steps in the viral life cycle and potential therapeutic targets. We previously reported that a human immunodeficiency virus type 2 (HIV-2) isolate was restricted to infection in some human cells, which led us to identify a step in the life cycle of HIV-2 detected after reverse transcription but prior to nuclear entry. The block is bypassed with a vesicular stomatitis virus glycoprotein G (VSV-G) envelope (A. McKnight et al., J. Virol. 75:6914-6922, 2001). We hypothesized that, although the restriction is apparent at a post-reverse transcription step, the lack of progress results from a failure of the virus to reach a cellular compartment with access to the nucleus. Here we analyzed molecular clones of the restricted virus, MCR, and an unrestricted virus, MCN. Using sequence analysis and gene swapping, we mapped the viral determinants to gag and env. Site-directed mutagenesis identified a single amino acid at position 207 in CA to be responsible for the gag restriction. Pseudotype experiments indicate that this step is also important for the infection of cells by HIV-1. The HIV-1 NL4.3 core is restricted if supplied with a restricted MCR envelope but not with VSV-G. Also the NL4.3 envelope rescues the restricted core of HIV-2 MCR. Abrogation experiments with MLV demonstrate that the restriction is distinct from Fv1/Ref1/Lv1. We propose that this represents a new lentiviral restriction, Lv2. Thus, the envelope and capsid of HIV act to ensure that the virus is delivered into an appropriate cellular compartment that allows postentry events in viral replication to proceed efficiently.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-10329384, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-10559316, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-10602376, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-11027299, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-11082317, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-11160732, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-11264352, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-11435571, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-11714623, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-11807237, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-11836433, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-12050374, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-12154227, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-12154231, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-12368468, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-12417576, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-12477877, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-12554640, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-12556682, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-12598123, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-12857888, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-1673040, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-1975844, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-2002549, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-2014240, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-3261635, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-4142387, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-6092693, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-7474093, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-7504934, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-7682627, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-7769705, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-8041786, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-8105392, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-8638416, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-8752279, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-8797814, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-9094670, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-9261346, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-9311787, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-9573226, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-9696790, http://linkedlifedata.com/resource/pubmed/commentcorrection/14747565-9841925
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
78
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2006-16
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:14747565-Animals, pubmed-meshheading:14747565-Capsid, pubmed-meshheading:14747565-Cell Line, pubmed-meshheading:14747565-Cloning, Molecular, pubmed-meshheading:14747565-Gene Expression Regulation, pubmed-meshheading:14747565-Gene Products, env, pubmed-meshheading:14747565-Gene Products, gag, pubmed-meshheading:14747565-HIV-1, pubmed-meshheading:14747565-HIV-2, pubmed-meshheading:14747565-HeLa Cells, pubmed-meshheading:14747565-Humans, pubmed-meshheading:14747565-Membrane Fusion, pubmed-meshheading:14747565-Mice, pubmed-meshheading:14747565-Molecular Sequence Data, pubmed-meshheading:14747565-Mutagenesis, Site-Directed, pubmed-meshheading:14747565-Proteins, pubmed-meshheading:14747565-Recombination, Genetic, pubmed-meshheading:14747565-Sequence Analysis, DNA, pubmed-meshheading:14747565-Virus Replication
pubmed:year
2004
pubmed:articleTitle
Lv2, a novel postentry restriction, is mediated by both capsid and envelope.
pubmed:affiliation
Wohl Virion Centre, Windeyer Institute of Medical Sciences, UCL, London W1T 4JF, United Kingdom.
pubmed:publicationType
Journal Article
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