| pubmed-article:147449 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:147449 | lifeskim:mentions | umls-concept:C1253959 | lld:lifeskim |
| pubmed-article:147449 | lifeskim:mentions | umls-concept:C0178539 | lld:lifeskim |
| pubmed-article:147449 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:147449 | lifeskim:mentions | umls-concept:C0006233 | lld:lifeskim |
| pubmed-article:147449 | lifeskim:mentions | umls-concept:C0204727 | lld:lifeskim |
| pubmed-article:147449 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:147449 | lifeskim:mentions | umls-concept:C0220781 | lld:lifeskim |
| pubmed-article:147449 | lifeskim:mentions | umls-concept:C1883254 | lld:lifeskim |
| pubmed-article:147449 | lifeskim:mentions | umls-concept:C0033268 | lld:lifeskim |
| pubmed-article:147449 | lifeskim:mentions | umls-concept:C0600210 | lld:lifeskim |
| pubmed-article:147449 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:147449 | pubmed:dateCreated | 1978-5-17 | lld:pubmed |
| pubmed-article:147449 | pubmed:abstractText | Incubation of human lymphoid cells with bromodeoxyuridine (BrdUrd) for short periods produces three classes of DNA containing analog: DNAHL (hybrid DNA, density approximately equal to 1.75 g/cm3), DNAint (intermediate density DNA, density approximately equal to 1.71 g/cm3), and DNAHH (DNA with both strands containing analog, density approximately equal to 1.80 g/cm3). Preparations of DNAint yield DNAHH after extensive shearing and/or treatment with single strand specific endonuclease. Cross-linking of pulse-labeled (BrdUrd + 3HdT) DNA in cells by treatment with trioxsalen and near UV light before lysis prevents the appearance of DNAHH.Cross-linking after lysis has little effect. A large fraction of DNAHH is obtained after incubation of cells with caffeine. Extraction of DNA at high salt concentration or cross-linking with trioxsalen and near UV light drastically reduced the amount of DNAHH obtained from caffeine-treated cells. We conclude that most DNAHH arises from in vitro branch migration in isolated DNA growing points. | lld:pubmed |
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| pubmed-article:147449 | pubmed:language | eng | lld:pubmed |
| pubmed-article:147449 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:147449 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:147449 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:147449 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:147449 | pubmed:issn | 0305-1048 | lld:pubmed |
| pubmed-article:147449 | pubmed:author | pubmed-author:StraussBB | lld:pubmed |
| pubmed-article:147449 | pubmed:author | pubmed-author:TatsumiKK | lld:pubmed |
| pubmed-article:147449 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:147449 | pubmed:volume | 5 | lld:pubmed |
| pubmed-article:147449 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:147449 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:147449 | pubmed:pagination | 331-47 | lld:pubmed |
| pubmed-article:147449 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:147449 | pubmed:year | 1978 | lld:pubmed |
| pubmed-article:147449 | pubmed:articleTitle | Production of DNA bifilarly substituted with bromodeoxyuridine in the first round of synthesis: branch migration during isolation of cellular DNA. | lld:pubmed |
| pubmed-article:147449 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:147449 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:147449 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
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