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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1978-5-17
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pubmed:abstractText |
Incubation of human lymphoid cells with bromodeoxyuridine (BrdUrd) for short periods produces three classes of DNA containing analog: DNAHL (hybrid DNA, density approximately equal to 1.75 g/cm3), DNAint (intermediate density DNA, density approximately equal to 1.71 g/cm3), and DNAHH (DNA with both strands containing analog, density approximately equal to 1.80 g/cm3). Preparations of DNAint yield DNAHH after extensive shearing and/or treatment with single strand specific endonuclease. Cross-linking of pulse-labeled (BrdUrd + 3HdT) DNA in cells by treatment with trioxsalen and near UV light before lysis prevents the appearance of DNAHH.Cross-linking after lysis has little effect. A large fraction of DNAHH is obtained after incubation of cells with caffeine. Extraction of DNA at high salt concentration or cross-linking with trioxsalen and near UV light drastically reduced the amount of DNAHH obtained from caffeine-treated cells. We conclude that most DNAHH arises from in vitro branch migration in isolated DNA growing points.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/147449-1065879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/147449-1066674,
http://linkedlifedata.com/resource/pubmed/commentcorrection/147449-1177959,
http://linkedlifedata.com/resource/pubmed/commentcorrection/147449-1250706,
http://linkedlifedata.com/resource/pubmed/commentcorrection/147449-1255724,
http://linkedlifedata.com/resource/pubmed/commentcorrection/147449-14284738,
http://linkedlifedata.com/resource/pubmed/commentcorrection/147449-4255472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/147449-4365578,
http://linkedlifedata.com/resource/pubmed/commentcorrection/147449-4416919,
http://linkedlifedata.com/resource/pubmed/commentcorrection/147449-4691350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/147449-4927248,
http://linkedlifedata.com/resource/pubmed/commentcorrection/147449-560256,
http://linkedlifedata.com/resource/pubmed/commentcorrection/147449-561393,
http://linkedlifedata.com/resource/pubmed/commentcorrection/147449-935855,
http://linkedlifedata.com/resource/pubmed/commentcorrection/147449-954106,
http://linkedlifedata.com/resource/pubmed/commentcorrection/147449-967189
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0305-1048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
331-47
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:147449-Bromodeoxyuridine,
pubmed-meshheading:147449-Caffeine,
pubmed-meshheading:147449-Cell Line,
pubmed-meshheading:147449-DNA,
pubmed-meshheading:147449-DNA Repair,
pubmed-meshheading:147449-DNA Replication,
pubmed-meshheading:147449-Floxuridine,
pubmed-meshheading:147449-Nucleic Acid Denaturation,
pubmed-meshheading:147449-Osmolar Concentration,
pubmed-meshheading:147449-Photochemistry,
pubmed-meshheading:147449-Trioxsalen
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pubmed:year |
1978
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pubmed:articleTitle |
Production of DNA bifilarly substituted with bromodeoxyuridine in the first round of synthesis: branch migration during isolation of cellular DNA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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