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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2004-1-27
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pubmed:abstractText |
Aurora-A kinase is frequently overexpressed/activated in human ovarian and breast cancers. A rat mammary tumor model study indicates that alterations of Aurora-A are early events during mammary tumor development (T. M. Goepfert et al., Cancer Res., 62: 4115-4122, 2002), suggesting that Aurora-A plays a pivotal role in transformation. However, the molecular mechanism by which Aurora-A induces ovarian and breast cell transformation remains elusive. Here we show that ectopic expression of Aurora-A induces telomerase activity in human ovarian and breast epithelial cell lines HIOSE118 and MCF-10A. The mRNA and promoter activities of human telomerase reverse transcriptase (hTERT) are stimulated by Aurora-A. Furthermore, we have demonstrated that the c-Myc binding sites of hTERT promoter are required for Aurora-A-induced hTERT promoter activity. Ectopic expression of Aurora-A up-regulates c-Myc. Knockdown of c-Myc by RNA interference attenuates Aurora-A-stimulated hTERT expression and telomerase activity. To our knowledge, these findings demonstrate, for the first time, that Aurora-A induces telomerase activity and hTERT by up-regulation of c-Myc and provides an additional mechanism for the role of Aurora-A in malignant transformation in addition to its cell cycle control.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Eg2 protein, Xenopus,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-myc,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Telomerase,
http://linkedlifedata.com/resource/pubmed/chemical/Xenopus Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/aurora kinase
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0008-5472
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
64
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
463-7
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pubmed:dateRevised |
2011-7-11
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pubmed:meshHeading |
pubmed-meshheading:14744757-Breast,
pubmed-meshheading:14744757-Cell Cycle Proteins,
pubmed-meshheading:14744757-Cell Line,
pubmed-meshheading:14744757-DNA Primers,
pubmed-meshheading:14744757-DNA-Binding Proteins,
pubmed-meshheading:14744757-Epithelial Cells,
pubmed-meshheading:14744757-Female,
pubmed-meshheading:14744757-Gene Expression Regulation,
pubmed-meshheading:14744757-Humans,
pubmed-meshheading:14744757-Kinetics,
pubmed-meshheading:14744757-Ovary,
pubmed-meshheading:14744757-Promoter Regions, Genetic,
pubmed-meshheading:14744757-Protein Kinases,
pubmed-meshheading:14744757-Protein-Serine-Threonine Kinases,
pubmed-meshheading:14744757-Proto-Oncogene Proteins c-myc,
pubmed-meshheading:14744757-RNA, Messenger,
pubmed-meshheading:14744757-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:14744757-Telomerase,
pubmed-meshheading:14744757-Transcription, Genetic,
pubmed-meshheading:14744757-Xenopus Proteins
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pubmed:year |
2004
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pubmed:articleTitle |
Aurora-A kinase regulates telomerase activity through c-Myc in human ovarian and breast epithelial cells.
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pubmed:affiliation |
Department of Pathology and Molecular Oncology Program, University of South Florida College of Medicine and H. Lee Moffitt Cancer Center and Research Institute, Tampa, Florida, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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