|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
2004-1-26
|
|
pubmed:abstractText |
Streptococcus pyogenes with null mutations in the csrRS regulatory locus are highly virulent in mice due to derepression of hyaluronic acid capsule synthesis and exotoxins, e.g., streptolysin S (SLS) and pyrogenic exotoxin B (SpeB). We generated derivatives of a DeltacsrRS strain that also carry deletions in hasAB (leading to an acapsular phenotype) or in sagA (phenotypically SLS-) or an interruption of speB (SpeB-) to test the relative contributions of these factors to the development of necrotic skin lesions. Inoculation of 2 x 10(6) to 4 x 10(6) CFU of either acapsular or SLS- strains into hairless mice resulted in lesions approximately 70% smaller than those of the DeltacsrRS parent strain. Elimination of SLS also reduced lethality from 100% to 0% at this inoculum (P < 10(-7); Fisher exact test). In contrast, SLS+ SpeB- mutants yielded lesions that were only 41% smaller than the parent strain (t = 2.2; P = 0.04), but only 3 the 17 lesions had dermal sloughing (P = 10(-5)). The nonulcerative lesions associated with SpeB- strains appeared pale with surrounding erythema. We conclude that capsule and SLS contribute to the subcutaneous spread of S. pyogenes and to a fatal outcome of infection. SpeB facilitates early dermal ulceration but has minor influence on lesion size and mortality. Large ulcerative lesions are observed only when both toxins are present.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/14742501-10368137,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14742501-10496909,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14742501-10497863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14742501-10768985,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14742501-10858242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14742501-11032859,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14742501-11035746,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14742501-11069243,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14742501-11069651,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14742501-11083799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14742501-11136470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14742501-11170990,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14742501-11237829,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14742501-11453703,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14742501-11517433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14742501-11598038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14742501-11740562,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14742501-11809255,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14742501-12065540,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14742501-12370433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14742501-3068490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14742501-310859,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14742501-6447727,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14742501-7516997,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14742501-7982988,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14742501-8093623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14742501-8355608,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14742501-8760820,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14742501-8890235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14742501-8975893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14742501-9169486,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14742501-9529097,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14742501-9673282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14742501-9691092,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14742501-9786197
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Feb
|
|
pubmed:issn |
0019-9567
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
72
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
623-8
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:14742501-Animals,
pubmed-meshheading:14742501-Bacterial Proteins,
pubmed-meshheading:14742501-Cysteine Endopeptidases,
pubmed-meshheading:14742501-Hyaluronic Acid,
pubmed-meshheading:14742501-Male,
pubmed-meshheading:14742501-Mice,
pubmed-meshheading:14742501-Mice, Hairless,
pubmed-meshheading:14742501-Repressor Proteins,
pubmed-meshheading:14742501-Skin Diseases, Infectious,
pubmed-meshheading:14742501-Streptococcal Infections,
pubmed-meshheading:14742501-Streptococcus pyogenes,
pubmed-meshheading:14742501-Streptolysins,
pubmed-meshheading:14742501-Virulence Factors
|
|
pubmed:year |
2004
|
|
pubmed:articleTitle |
Contribution of CsrR-regulated virulence factors to the progress and outcome of murine skin infections by Streptococcus pyogenes.
|
|
pubmed:affiliation |
Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan 48109-0378, USA. cengleb@umich.edu
|
|
pubmed:publicationType |
Journal Article
|
