|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
2004-1-23
|
|
pubmed:abstractText |
Inhibition of co-stimulatory signals for T cells by interrupting CD80/CD86-CD28 and CD40-CD154 interactions is a promising approach to prevent transplant rejection and to induce graft tolerance. However, this tolerizing treatment might affect T cell reactivity towards all the antigens to which the immune system is exposed during treatment. We addressed the question whether such inhibition of co-stimulatory ligands on human antigen presenting cells (APC) would affect T cell reactivity against a virus. This was tested in an in vitro system with freshly isolated human monocytes transduced with adenovirus (ad) containing either murine interferon-gamma (mIFN-gamma) or green fluorescent protein (GFP) as marker transgene. T cells co-cultured with transduced monocytes proliferated and produced cytokines. These 'primed' T cells had strong antiviral activity as they subsequently killed ad/GFP-transduced monocytes and reduced mIFN-gamma accumulation in coculture with ad/mIFN-transduced monocytes. However, if priming had occurred in the presence of blocking anti-CD40/CD80/CD86 MoAbs, generation of this antiviral activity was completely prevented. Moreover, T cells primed in the absence of co-stimulatory cells failed to proliferate upon restimulation with adenovirus-transduced monocytes. The results confirm that co-stimulatory signals from APC are required for efficient induction of antiviral T cell activity and point to a potential infectious risk of blocking co-stimulatory signals.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/14738453-10361876,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14738453-10458748,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14738453-10590107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14738453-10742157,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14738453-10906225,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14738453-11407903,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14738453-11781244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14738453-11973629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14738453-12176880,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14738453-12183158,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14738453-12640302,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14738453-12705853,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14738453-1280221,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14738453-3263438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14738453-4358125,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14738453-7504989,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14738453-7507511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14738453-7510232,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14738453-7515910,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14738453-7520767,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14738453-7550348,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14738453-7686564,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14738453-8486235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14738453-8632801,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14738453-8671587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14738453-9010719,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14738453-9114052,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14738453-9144487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14738453-9238056,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14738453-9414251,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14738453-9422428,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14738453-9624005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14738453-9696851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/14738453-9743331
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Blocking,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD40,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD80,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD86,
http://linkedlifedata.com/resource/pubmed/chemical/Biological Markers,
http://linkedlifedata.com/resource/pubmed/chemical/CD86 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Green Fluorescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Indicators and Reagents,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-13,
http://linkedlifedata.com/resource/pubmed/chemical/Luminescent Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Feb
|
|
pubmed:issn |
0009-9104
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
135
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
253-8
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:14738453-Humans,
pubmed-meshheading:14738453-Female,
pubmed-meshheading:14738453-Indicators and Reagents,
pubmed-meshheading:14738453-Male,
pubmed-meshheading:14738453-Monocytes,
pubmed-meshheading:14738453-Adult,
pubmed-meshheading:14738453-Cell Division,
pubmed-meshheading:14738453-Adenoviridae,
pubmed-meshheading:14738453-Middle Aged,
pubmed-meshheading:14738453-Cells, Cultured,
pubmed-meshheading:14738453-Immune Tolerance,
pubmed-meshheading:14738453-Lymphocyte Activation,
pubmed-meshheading:14738453-Transduction, Genetic,
pubmed-meshheading:14738453-Biological Markers,
pubmed-meshheading:14738453-T-Lymphocytes,
pubmed-meshheading:14738453-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:14738453-Antibodies, Blocking,
pubmed-meshheading:14738453-Antigen-Presenting Cells,
pubmed-meshheading:14738453-Antigens, CD,
pubmed-meshheading:14738453-Membrane Glycoproteins,
pubmed-meshheading:14738453-Luminescent Proteins,
pubmed-meshheading:14738453-Interferon-gamma,
pubmed-meshheading:14738453-Transgenes,
pubmed-meshheading:14738453-Antigens, CD40
|
