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pubmed:abstractText |
The GT1 cell has been widely used as a model cell to study cellular functions of GnRH neurons. Despite the importance of Ca(2+) channels, little is known except for L- and T-type Ca(2+) channels in GT1 cells. Therefore, we studied the diversity of voltage-gated Ca(2+) channels in GT1-7 cells with perforated-patch clamp and RT-PCR. An R-type Ca(2+) channel blocker, SNX-482, inhibited the Ca(2+) currents by 75.6% in all cells examined (n = 9). A T-type Ca(2+) channel blocker, Ni(2+), inhibited the Ca(2+) currents by 12.6% in all cells examined (n = 9). An L-type Ca(2+) channel blocker, nimodipine, inhibited the Ca(2+) currents by 17.9% in five of 11 cells examined. When using Ba(2+) as a charge carrier, another dihydropyridine antagonist, nifedipine, clearly inhibited the currents by 12.1% in all cells examined (n = 16). An N-type Ca(2+) channel blocker, omega-conotoxin-GVIA, inhibited the Ca(2+) currents by 13.8% in three of 20 cells examined. A P/Q type Ca(2+) channel blocker, omega-agatoxin-IVA, had no effect on the currents (n = 9). RT-PCR revealed that GT1-7 cells expressed the alpha(1B), alpha(1D), alpha(1E), and alpha(1H) subunit mRNA. Furthermore, SNX-482 and nifedipine inhibited the high K(+)-induced increase in the intracellular Ca(2+) concentration and GnRH release. omega-Conotoxin-GVIA and omega-agatoxin-IVA had no effect. These results suggest that GT1-7 cells express R-, L-, N-, and T-type voltage-gated Ca(2+) channels; the R-type was a major current component, and the L-, N-, and T-types were minor ones. The R- and L-type Ca(2+) channels play a critical role in the regulation of Ca(2+)-dependent GnRH release.
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