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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
|
| pubmed:dateCreated |
1993-2-1
|
| pubmed:abstractText |
During a sero-survey for lymphotropic retroviruses, seven screening tests were performed for the three lymphotropic retroviruses of relevance in Djibouti, East Africa (HIV-1, HIV-2 and HTLV-1). Of the 82 subjects whose sera reacted in at least one retroviral screening assay, about one third could be followed, and their sera were re-examined after a 5-month interval, and then after an additional 3-month interval. Six selected individuals are reported here, whose retroviral serologies presented important and often unexplained changes over an eight-month period. The six cases summarize prototypic situations and present serological results in a style appropriate to stimulate thought on the significance and interpretation of lymphotropic viral serologies. Each case study is followed by a set of questions that formulate pertinent serological concepts.
|
| pubmed:keyword |
http://linkedlifedata.com/resource/pubmed/keyword/Acquired Immunodeficiency Syndrome,
http://linkedlifedata.com/resource/pubmed/keyword/Africa,
http://linkedlifedata.com/resource/pubmed/keyword/Africa South Of The Sahara,
http://linkedlifedata.com/resource/pubmed/keyword/Arab Countries,
http://linkedlifedata.com/resource/pubmed/keyword/Case Histories,
http://linkedlifedata.com/resource/pubmed/keyword/Data Collection,
http://linkedlifedata.com/resource/pubmed/keyword/Developing Countries,
http://linkedlifedata.com/resource/pubmed/keyword/Diseases,
http://linkedlifedata.com/resource/pubmed/keyword/Djibouti,
http://linkedlifedata.com/resource/pubmed/keyword/Eastern Africa,
http://linkedlifedata.com/resource/pubmed/keyword/Examinations And Diagnoses,
http://linkedlifedata.com/resource/pubmed/keyword/Follow-up Studies,
http://linkedlifedata.com/resource/pubmed/keyword/French Speaking Africa,
http://linkedlifedata.com/resource/pubmed/keyword/Hiv Infections,
http://linkedlifedata.com/resource/pubmed/keyword/Hiv Serodiagnosis,
http://linkedlifedata.com/resource/pubmed/keyword/Laboratory Examinations And Diagnoses,
http://linkedlifedata.com/resource/pubmed/keyword/Population At Risk,
http://linkedlifedata.com/resource/pubmed/keyword/Research Methodology,
http://linkedlifedata.com/resource/pubmed/keyword/Sampling Studies,
http://linkedlifedata.com/resource/pubmed/keyword/Studies,
http://linkedlifedata.com/resource/pubmed/keyword/Surveys,
http://linkedlifedata.com/resource/pubmed/keyword/Viral Diseases
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0012-835X
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
69
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
563-5
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:otherAbstract |
PIP: A total of 600 sera were collected in the city of Djibouti during a national HIV survey in June 1988 from individuals at high risk for acquiring HIV-1. 7 screening tests were performed for antibodies to the 3 lymphotropic retroviruses HIV-1, HIV-2, and HTLV-1. 82 subjects' sera reacted in at least 1 retroviral screening assay. Sequential serological test outcomes are presented for the 6 cases whose retroviral serologies after a 5-month interval in November 1988, and after an additional 3-month interval in February 1989 presented often unexplained changes. Each case study is followed by a set of questions amounting to 26 diagnostic problems. During the follow-ups, a 10 ml venous blood sample of the 6 subjects was used to separate and process mononuclear cells for phenotypic analysis and polymerase chain reaction (PCR). Western Blot testing for the 3 retroviruses was performed on patients' plasma. A neutralization enzyme-linked immunosorbent assay to detect circulating HIV antigen in sera was also performed on all samples. Case 1 was a false positive HIV-1 screening assay in a 22-year old unmarried heterosexual male with a sexually transmitted disease. The patient's serum tested negative by 6 tests, but positive by the ELAVIA, which gave 1 nonrepeatable reactive result for HIV-1. PCR analysis was strongly positive, despite the still indeterminate profile of the Western Blot. The assay that gave 1 single (false?) positive result was perhaps the only assay that detected an early HIV-1 infection, or the PCR result may have been false positive. Case 2 was a triple sero-conversion and co-infection in a female prostitute with an indeterminate HIV-1 Western Blot. Case 2 was another prostitute, with HIV Western Blot sero-progression or sero-repression. Case 4 was a delayed Western Blot sero-conversion in a female with an early positive HTLV-1 screening test. Cases 5 and 6 were 2 female prostitutes with antibodies against either envelope or polymerase antigens alone. The dilemma is what the physician should tell the person with a question ale lymphotropic serology.
|
| pubmed:meshHeading |
pubmed-meshheading:1473510-Adult,
pubmed-meshheading:1473510-Blotting, Western,
pubmed-meshheading:1473510-Djibouti,
pubmed-meshheading:1473510-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:1473510-Female,
pubmed-meshheading:1473510-HIV Infections,
pubmed-meshheading:1473510-HIV Seroprevalence,
pubmed-meshheading:1473510-HIV-1,
pubmed-meshheading:1473510-HIV-2,
pubmed-meshheading:1473510-HTLV-I Infections,
pubmed-meshheading:1473510-Humans,
pubmed-meshheading:1473510-Male,
pubmed-meshheading:1473510-Mass Screening,
pubmed-meshheading:1473510-Polymerase Chain Reaction,
pubmed-meshheading:1473510-Prostitution,
pubmed-meshheading:1473510-Risk Factors,
pubmed-meshheading:1473510-Seroepidemiologic Studies
|
| pubmed:year |
1992
|
| pubmed:articleTitle |
Diagnostic challenges: lymphotropic sero-"questionables".
|
| pubmed:affiliation |
U.S. Naval Medical Research Unit No. 3, Cairo, Egypt.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Case Reports
|