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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
17
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pubmed:dateCreated |
2004-4-19
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pubmed:abstractText |
Terminal differentiation of hematopoietic cells follows a precisely orchestrated program of transcriptional regulatory events at the promoters of both lineage-specific and ubiquitous genes. Here we show that the transcription factor ATF2 is associated with the induction of granulocytic differentiation, and the molecular interaction of ATF2 with a tissue-specific coactivator activating signal cointegator-2 (ASC-2) potentiates the differentiation procedure. All-trans retinoic acid (RA) induced the phosphorylation and expression of ATF2 in the early and middle phase of granulocyte differentiation, respectively. The activation of granulocyte-specific gene expression is increased with the concerted action of another basic regionleucine zipper factor, CCAAT/enhancer-binding protein (C/EBPalpha), and ASC-2, which function in a cooperative manner. The interaction between ATF2 and C/EBPalpha in RA-treated cells was enhanced by the ectopic expression of ASC-2. ATF2-mediated transactivation was also increased by co-transfection of ASC-2. This resulted from the direct protein interaction that the N-terminal transactivation domain of ATF2 interacts with the central region of ASC-2. Furthermore, the molecular interaction of ATF2 and ASC-2 was stimulated by RA treatment and inhibited by p38beta kinase inhibitor. Taking these results together, these results suggest that the differentiation-dependent expression and phosphorylation of ATF2 protein physically and functionally interacts with C/EBPalpha and coativator ASC-2 and synergizes to induce target gene transcription during granulocytic differentiation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ATF2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Activating Transcription Factor 2,
http://linkedlifedata.com/resource/pubmed/chemical/CCAAT-Enhancer-Binding Protein-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/Chromatin,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP Response...,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Glutathione Transferase,
http://linkedlifedata.com/resource/pubmed/chemical/Luciferases,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinase 11,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Tretinoin
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
23
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pubmed:volume |
279
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
16996-7003
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:14734562-Activating Transcription Factor 2,
pubmed-meshheading:14734562-Amino Acid Motifs,
pubmed-meshheading:14734562-Blotting, Western,
pubmed-meshheading:14734562-CCAAT-Enhancer-Binding Protein-alpha,
pubmed-meshheading:14734562-Cell Differentiation,
pubmed-meshheading:14734562-Cell Line,
pubmed-meshheading:14734562-Cell Lineage,
pubmed-meshheading:14734562-Chromatin,
pubmed-meshheading:14734562-Cyclic AMP Response Element-Binding Protein,
pubmed-meshheading:14734562-Dose-Response Relationship, Drug,
pubmed-meshheading:14734562-Enzyme Inhibitors,
pubmed-meshheading:14734562-Genes, Dominant,
pubmed-meshheading:14734562-Glutathione Transferase,
pubmed-meshheading:14734562-Granulocytes,
pubmed-meshheading:14734562-HeLa Cells,
pubmed-meshheading:14734562-Humans,
pubmed-meshheading:14734562-Luciferases,
pubmed-meshheading:14734562-Mitogen-Activated Protein Kinase 11,
pubmed-meshheading:14734562-Mitogen-Activated Protein Kinases,
pubmed-meshheading:14734562-Mutation,
pubmed-meshheading:14734562-Phosphorylation,
pubmed-meshheading:14734562-Precipitin Tests,
pubmed-meshheading:14734562-Protein Binding,
pubmed-meshheading:14734562-Protein Structure, Tertiary,
pubmed-meshheading:14734562-Time Factors,
pubmed-meshheading:14734562-Transcription, Genetic,
pubmed-meshheading:14734562-Transcription Factors,
pubmed-meshheading:14734562-Transcriptional Activation,
pubmed-meshheading:14734562-Transfection,
pubmed-meshheading:14734562-Tretinoin,
pubmed-meshheading:14734562-Two-Hybrid System Techniques,
pubmed-meshheading:14734562-U937 Cells
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pubmed:year |
2004
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pubmed:articleTitle |
Activation and interaction of ATF2 with the coactivator ASC-2 are responsive for granulocytic differentiation by retinoic acid.
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pubmed:affiliation |
Department of Molecular Biology, College of Natural Sciences, Pusan National University, Busan 609-735, Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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